Construction of a Genetic Linkage Map in Tetraploid Species Using Molecular Markers
| Autor: | J. E. Bradshaw, Dan Milbourne, James W. McNicol, Christine A. Hackett, Zewei Luo |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Genetic Markers
Genotype Genetic Linkage Population Biology Genetic linkage Genetics Cluster Analysis Allele education Gene Alleles Crosses Genetic Genes Dominant Solanum tuberosum Recombination Genetic Linkage (software) Likelihood Functions education.field_of_study Models Statistical Ploidies Models Genetic Chromosome Mapping Phenotype Genetic marker Amplified fragment length polymorphism Lod Score Algorithms Research Article |
| Zdroj: | Genetics. 157:1369-1385 |
| ISSN: | 1943-2631 |
| DOI: | 10.1093/genetics/157.3.1369 |
| Popis: | This article presents methodology for the construction of a linkage map in an autotetraploid species, using either codominant or dominant molecular markers scored on two parents and their full-sib progeny. The steps of the analysis are as follows: identification of parental genotypes from the parental and offspring phenotypes; testing for independent segregation of markers; partition of markers into linkage groups using cluster analysis; maximum-likelihood estimation of the phase, recombination frequency, and LOD score for all pairs of markers in the same linkage group using the EM algorithm; ordering the markers and estimating distances between them; and reconstructing their linkage phases. The information from different marker configurations about the recombination frequency is examined and found to vary considerably, depending on the number of different alleles, the number of alleles shared by the parents, and the phase of the markers. The methods are applied to a simulated data set and to a small set of SSR and AFLP markers scored in a full-sib population of tetraploid potato. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|