Evaluation of Congo Red Staining in Degenerating Porcine Photoreceptors In Vitro: Protective Effects by Structural and Trophic Support
| Autor: | Kjell Johansson, Dick Delbro, Anders Kvanta, Camilla Mohlin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Opsin Programmed cell death Histology genetic structures Swine Inclusion bodies Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Microscopy Electron Transmission medicine Extracellular Animals Humans Coloring Agents Retina Retinal pigment epithelium Staining and Labeling Retinal Degeneration Congo Red Articles Coculture Techniques Cell biology Congo red Staining 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence chemistry sense organs Anatomy 030217 neurology & neurosurgery Photoreceptor Cells Vertebrate |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 66:631-641 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1369/0022155418768222 |
| Popis: | Congo red (CR) is a histological stain used for the detection of extracellular amyloids mediating various neurodegenerative diseases. Given that damaged photoreceptors appear to degenerate similarly to other nerve cells, CR staining was evaluated in experimentally injured porcine retina. CR staining appeared mostly as discrete cytosolic deposits with no obvious plaque formation during the investigated time period. Increases of CR labeling coincided temporally with the known accumulation of mislocalized opsins and increases of cell death. Coculture, either with human retinal pigment epithelium (ARPE) or human neural progenitor (ReN) cells, was accompanied by a significant reduction of CR labeling. Of particular interest was the reduction of CR labeling in cone photoreceptors, which are important for the perception of color and fine details and afflicted in age-related macular degeneration (AMD). Electron microscopy revealed inclusions in the inner segment, cell body, and occasionally synaptic terminals of photoreceptor cells in cultured specimens. Closer examinations indicated the presence of different types of inclusions resembling protein aggregates as well as inclusion bodies. The current results indicate that injury-related response resulted in accumulation of CR deposits in photoreceptor cells, and that trophic and/or structural support attenuated this response. |
| Databáze: | OpenAIRE |
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