High-speed compressed-sensing fluorescence lifetime imaging microscopy of live cells
| Autor: | Young Jae Lee, Yayao Ma, Liang Gao, Catherine Best-Popescu |
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| Rok vydání: | 2021 |
| Předmět: |
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Fluorescence-lifetime imaging microscopy Large field of view Microscopy FLIM Multidisciplinary Materials science business.industry Lasers Bioengineering Biological Sciences Photobleaching Fluorescence Molecular Imaging Optics Compressed sensing Single camera high-speed Microscopy Fluorescence compressed imaging Humans business Excitation |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol 118, iss 3 Proc Natl Acad Sci U S A |
| Popis: | We present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of live cells based on a compressed sensing scheme. By leveraging the compressibility of biological scenes in a specific domain, we simultaneously record the time-lapse fluorescence decay upon pulsed laser excitation within a large field of view. The resultant system, referred to as compressed FLIM, can acquire a widefield fluorescence lifetime image within a single camera exposure, eliminating the motion artifact and minimizing the photobleaching and phototoxicity. The imaging speed, limited only by the readout speed of the camera, is up to 100 Hz. We demonstrated the utility of compressed FLIM in imaging various transient dynamics at the microscopic scale. |
| Databáze: | OpenAIRE |
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