IL-21 promotes the expansion of CD27+CD28+ tumor infiltrating lymphocytes with high cytotoxic potential and low collateral expansion of regulatory T cells
Autor: | Daniel J. Powell, Erik Hooijberg, Annelies W. Turksma, Tanja D. de Gruijl, S. Albelda, Megan M. Suhoski, Alfons J.M. van den Eertwegh, Anita G.M. Stam, Carl H. June, Winald R. Gerritsen, Rik J. Scheper, Saskia J. A. M. Santegoets |
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Přispěvatelé: | Medical oncology laboratory, Pathology, Medical oncology, CCA - Immuno-pathogenesis |
Rok vydání: | 2013 |
Předmět: |
Adoptive cell transfer
Biopsy medicine.medical_treatment T cell Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Biology T-Lymphocytes Regulatory General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Lymphocytes Tumor-Infiltrating 0302 clinical medicine CD28 Antigens Immune Regulation [NCMLS 2] Neoplasms medicine Humans Cytotoxic T cell Cell Proliferation 030304 developmental biology Medicine(all) 0303 health sciences Biochemistry Genetics and Molecular Biology(all) Tumor-infiltrating lymphocytes Research Interleukins Receptors IgG CD28 General Medicine Flow Cytometry Autologous tumor cell Tumor Necrosis Factor Receptor Superfamily Member 7 Phenotype Cytokine medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Cytokines K562 Cells CD8 |
Zdroj: | Journal of Translational Medicine, 11, pp. 37 Journal of Translational Medicine, 11, 37-37. BioMed Central Journal of Translational Medicine Santegoets, S J A M, Turksma, A W, Suhoski, M M, Stam, A G M, Albelda, S M, Hooijberg, E, Scheper, R J, van den Eertwegh, A J M, Gerritsen, W R, Powell, D J J, June, C H & de Gruijl, T D 2013, ' IL-21 promotes the expansion of CD27+CD28+ tumor infiltrating lymphocytes with high cytotoxic potential and low collateral expansion of regulatory T cells ', Journal of Translational Medicine, vol. 11, pp. 37-37 . https://doi.org/10.1186/1479-5876-11-37 Journal of Translational Medicine, 11, 37 |
ISSN: | 1479-5876 |
Popis: | Contains fulltext : 118572.pdf (Publisher’s version ) (Open Access) BACKGROUND: Adoptive cell transfer of tumor infiltrating lymphocytes has shown clinical efficacy in the treatment of melanoma and is now also being explored in other tumor types. Generation of sufficient numbers of effector T cells requires extensive ex vivo expansion, often at the cost of T cell differentiation and potency. For the past 20 years, IL-2 has been the key cytokine applied in the expansion of TIL for ACT. However, the use of IL-2 has also led to collateral expansion of regulatory T cells (Tregs) and progressive T cell differentiation, factors known to limit in vivo persistence and activity of transferred TIL. The use of alternative T cell growth factors is therefore warranted. Here, we have compared the effects of IL-2, -15 and -21 cytokines on the expansion and activation of TIL from single-cell suspensions of non-small cell lung cancer, ovarian cancer and melanoma. METHODS: We applied the K562-based artificial APC (aAPC) platform for the direct and rapid expansion of tumor infiltrating lymphocytes isolated from primary cancer specimens. These aAPC were engineered to express the Fc-gamma receptor CD32 (for anti-CD3 antibody binding), the co-stimulatory molecule 4-1BBL, and to secrete either IL-2, IL-15 or IL-21 cytokine. RESULTS: Although IL-2 aAPC induced the greatest overall TIL expansion, IL-21 aAPC induced superior expansion of CD8+ T cells with a CD27+CD28+ "young" phenotype and superior functional cytotoxic effector characteristics, without collateral expansion of Tregs. CONCLUSION: Our data rationalize the clinical application of IL-21-secreting aAPC as a standardized cell-based platform in the expansion of "young" effector TIL for ACT. |
Databáze: | OpenAIRE |
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