Corn oil versus lard: Metabolic effects of omega-3 fatty acids in mice fed obesogenic diets with different fatty acid composition
| Autor: | Jana Pavlisova, Eva Tvrzická, Barbora Stankova, Martin Rossmeisl, Jan Kopecky, Kristina Bardova |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Dietary lipid 030209 endocrinology & metabolism Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Insulin resistance Fatty Acids Omega-6 Internal medicine Fatty Acids Omega-3 medicine Animals chemistry.chemical_classification food and beverages General Medicine medicine.disease Dietary Fats Eicosapentaenoic acid Fatty Liver 030104 developmental biology Endocrinology Adipose Tissue chemistry Docosahexaenoic acid lipids (amino acids peptides and proteins) Corn Oil Insulin Resistance Steatosis Stearoyl-CoA desaturase-1 Corn oil Polyunsaturated fatty acid |
| Zdroj: | Biochimie. 124:150-162 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/j.biochi.2015.07.001 |
| Popis: | Mixed results have been obtained regarding the level of insulin resistance induced by high-fat diets rich in saturated fatty acids (SFA) when compared to those enriched by polyunsaturated fatty acids (PUFA), and how metabolic effects of marine PUFA of n-3 series, i.e. docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), depend on dietary lipid background. Here we compared two high-fat diets, in which the major lipid constituent was based either on SFA in the form of pork lard (LHF diet) or PUFA of n-6 series (Omega-6) as corn oil (cHF diet). Both cHF and LHF parental diets were also supplemented with EPA+DHA (∼30 g/kg diet) to produce cHF+F and LHF+F diet, respectively. Male C57BL/6N mice were fed the experimental diets for 8 weeks. Insulin sensitivity was assessed by hyperinsulinemic-euglycemic clamps in mice fed LHF and cHF diets, and then metabolic effects of cHF+F and LHF+F diets were assessed focusing on the liver and epididymal white adipose tissue (eWAT). Both LHF and cHF induced comparable weight gain and the level of insulin resistance, however LHF-fed mice showed increased hepatic steatosis associated with elevated activity of stearoyl-CoA desaturase-1 (SCD1), and lower plasma triacylglycerol levels when compared to cHF. Despite lowering hepatic SCD1 activity, which was concomitant with reduced hepatic steatosis reaching the level observed in cHF+F mice, LHF+F did not decrease adiposity and the weight of eWAT, and rather further impaired insulin sensitivity relative to cHF+F, that tended to improve it. In conclusion, high-fat diets containing as much as ∼35 weight% as lipids induce similar weight gain and impairment of insulin sensitivity irrespective whether they are based on SFA or Omega-6. Although the SFA-rich diet containing EPA+DHA efficiently reduced hepatic steatosis, it did so without a corresponding improvement in insulin sensitivity and in the absence of effect on adiposity. |
| Databáze: | OpenAIRE |
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