Prognostic Significance of MAGE-A11 in Esophageal Squamous Cell Carcinoma and Identification of Related Genes Based on DNA Microarray
| Autor: | Yishui Lian, Weina Huang, Fei Liu, Meixiang Sang, Shina Liu, Lina Gu, Baoen Shan, Danjing Yin, Xiaojie Fan, Chunyan Ding |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male endocrine system Pathology medicine.medical_specialty Microarray Esophageal Neoplasms Gene Expression Biology Metastasis 03 medical and health sciences Antigens Neoplasm Gene expression medicine Humans MTT assay neoplasms Oligonucleotide Array Sequence Analysis Cell growth General Medicine Middle Aged medicine.disease Prognosis Immunohistochemistry digestive system diseases Protein ubiquitination Neoplasm Proteins 030104 developmental biology Lymphatic Metastasis Cancer research Carcinoma Squamous Cell Female Esophageal Squamous Cell Carcinoma DNA microarray |
| Zdroj: | Archives of medical research. 47(3) |
| ISSN: | 1873-5487 |
| Popis: | Background and Aims This study aims to investigate the expression pattern of melanoma-associated antigen-A11 (MAGE-A11) in esophageal squamous cell carcinoma (ESCC) specimens and analyze its prognostic significance for ESCC patients. In addition, the purpose of our study was also to explore the biological function of MAGE-A11 in ESCC cells based on DNA microarray. Methods Immunohistochemistry was used to detect the expression of MAGE-A11 in ESCC specimens, and its prognostic significance was analyzed by statistical analysis. DNA microarray and quantitative RT-PCR were used to explore the different expression of MAGE-A11 downstream genes in ESCC cells. Cell invasion assay and MTT assay were used to detect the effect of MAGE-A11 cDNA on the invasion and proliferation of ESCC cells. Results Of the ESCC specimens, 59.3% showed positive MAGE-A11 expression. MAGE-A11 expression in ESCC specimens was positively associated with distant lymph node metastasis. Overall survival of ESCC patients with positive MAGE-A11 expression was shorter than in patients with negative MAGE-A11 expression. Multivariate Cox regression analysis showed MAGE-A11 expression is an independent poor prognostic factor for ESCC patients. Overexpression of MAGE-A11 changed a variety of gene expressions, which was associated with various cell functions such as protein ubiquitination, cell proliferation and apoptosis, tumor invasion and metastasis. Overexpression of MAGE-A11 directly increased the invasion and proliferation of ESCC cells. Conclusions MAGE-A11 is an independent poor prognostic marker for ESCC patients. MAGE-A11 regulates various cell functions and directly increases the invasion and proliferation of ESCC cells. |
| Databáze: | OpenAIRE |
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