Biodiversity and full genome sequence of potato viruses Alfalfa mosaic virus and potato leaf roll virus in Egypt
| Autor: | Faiza A. Fattouh, Radwa M. Taha, Engy E. Abdel Aleem |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine viruses Genome Viral Biology 01 natural sciences General Biochemistry Genetics and Molecular Biology Virus Crop 03 medical and health sciences Alfalfa mosaic virus Plant virus Solanum tuberosum Whole genome sequencing Genetic diversity Microbiota fungi Nucleic acid sequence food and beverages biology.organism_classification Virology Luteoviridae 030104 developmental biology Potato virus Y Egypt 010606 plant biology & botany |
| Zdroj: | Zeitschrift für Naturforschung C. 73:423-438 |
| ISSN: | 1865-7125 0939-5075 |
| DOI: | 10.1515/znc-2018-0033 |
| Popis: | Solanum tuberosum (potato) is the second most important vegetable crop in Egypt. It is locally consumed, manufactured or supplied for export to Europe and other Arab countries. Potato is subject to infection by a number of plant viruses, which affect its yield and quality. Potato virus Y (PVY), potato leaf roll virus (PLRV), and Alfalfa mosaic virus (AMV) were detected in major potato-growing areas surveyed. Multiplex-RT-PCR assay was used for the detection of these three viruses in one reaction using three specific primer pairs designed to amplify genomic parts of each virus (1594 bp for PLRV, 795 bp for AMV, 801 bp for PVY). All three viruses were detected in a single reaction mixture in naturally infected field-grown potatoes. Multiplex RT-PCR improved sensitivity necessary for the early detection of infection. Incidence of single, double, or triple infection has been recorded in some locations. Full-length sequencing has been performed for an Egyptian FER isolate of PLRV. Through phylogenetic analysis, it was shown to occupy the same clade with isolate JokerMV10 from Germany. Complete nucleotide sequence of an Egyptian FER isolate of AMV and phylogenetic analysis was also performed; we propose that it is a new distinct strain of AMV belonging to a new subgroup IIC. This is the first complete nucleotide sequence of an Egyptian isolate of AMV. Genetic biodiversity of devastating potato viruses necessitates continuous monitoring of new genetic variants of such viruses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|