Rat mesenchymal stem cell cultures as a model to elucidate the cellular and molecular pathogenesis of bone metaplasia induced by Solanum glaucophyllum intoxication
Autor: | Eduardo Juan Gimeno, Marília Martins Melo, Lorenna Alves Gomes, Kênia Mara M.C. Cardoso, Fabrício Gomes Melo, Ana Flávia Machado Botelho, Amanda Maria Sena Reis, Adriana Raquel Massone, Natália de Melo Ocarino, Rogéria Serakides |
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Rok vydání: | 2019 |
Předmět: |
Solanum glaucophyllum
Bone Morphogenetic Protein 2 Rat Bone Marrow Matrix (biology) Toxicology Bone and Bones Bone Metaplasia Toxicity Tests medicine Animals Integrin-Binding Sialoprotein Metaplasia biology Chemistry Plant Extracts Mesenchymal stem cell Molecular pathogenesis Mesenchymal Stem Cells biology.organism_classification Molecular biology Rats medicine.anatomical_structure Osteopontin Bone marrow Stem cell Chromatography Liquid |
Zdroj: | Toxicon : official journal of the International Society on Toxinology. 169 |
ISSN: | 1879-3150 |
Popis: | The hypothesis of this experiment is that mesenchymal stem cells (MSCs) are involved in the genesis of the bone metaplasia caused by Solanum glaucophyllum intoxication. We determined using liquid chromatography that 1 mL of plant extract contained 3.8 μl of 1,25(OH)2D3. The ability of 100 μL, 1 mL and 5 mL of extract/L, containing 1 nM (0.4 μg/L), 10 nM (4 μg/L) and 50 nM (20 μg/L) of 1,25(OH)2D3, respectively, in inducing the osteogenic differentiation in bone marrow MSCs from rats was tested. At the concentrations of 1 and 5 mL of extract/L of culture medium without osteogenesis-inducing factors, the plant extract induced the osteogenic differentiation of the MSCs, as was evidenced by the greater synthesis of mineralized matrix. At the higher concentration (5 mL of extract/L), an increase in the relative expression of BMP-2 gene was observed. It was concluded that rat bone marrow MSC culture is a good model for studying the effects of the S. glaucophyllum extract on the osteogenic differentiation of undifferentiated cells. Also, S. glaucophyllum extracts containing 10 nM (4 μg/L) and 50 nM (20 μg/L) of 1,25(OH)2D3 induce the osteogenic differentiation of MSCs, suggesting that this is one of the mechanisms by which S. glaucophyllum causes bone metaplasia. |
Databáze: | OpenAIRE |
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