miR-483-3p regulates osteogenic differentiation of bone marrow mesenchymal stem cells by targeting STAT1
| Autor: | Wen‑Feng Xiao, Qi Guo, Ying Yuan, Ye Xiao, Guang‑Wei Wang, Tie Jian Jiang, Li Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Transcription Genetic Cellular differentiation Cell osteogenic differentiation Bone Marrow Cells Core Binding Factor Alpha 1 Subunit Bone morphogenetic protein Biochemistry Mice 03 medical and health sciences 0302 clinical medicine STAT1 stomatognathic system Osteogenesis microRNA Genetics medicine Animals Molecular Biology Transcription factor Regulation of gene expression Oncogene Chemistry Cell Differentiation Mesenchymal Stem Cells Articles microRNA-483-3p Cell biology RUNX2 MicroRNAs STAT1 Transcription Factor 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Oncology 030220 oncology & carcinogenesis Molecular Medicine Female |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is regulated by a variety of intracellular regulatory factors including osterix, runt‑related transcription factor 2 (RUNX2), bone morphogenetic proteins and transforming growth factorβ. Recent studies have shown that microRNAs (miRs) serve a crucial role in this process. In the present study, miR‑483‑3p levels were significantly increased during osteogenic differentiation of mouse and human BMSCs. Overexpression of miR‑483‑3p promoted osteogenic differentiation, whereas inhibition of miR‑483‑3p reversed these effects. miR‑483‑3p regulated osteogenic differentiation of BMSCs by targeting STAT1, and thus enhancing RUNX2 transcriptional activity and RUNX2 nuclear translocation. In vivo, overexpression of miR‑483‑3p using a BMSC‑specific aptamer delivery system stimulated bone formation in aged mice. Therefore, the present study suggested that miR‑483‑3p promoted osteogenic differentiation of BMSCs by targeting STAT1, and miR‑483‑3 prepresent a potential therapeutic target for age‑related bone loss. |
| Databáze: | OpenAIRE |
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