Automated Method for the Determination of 5′-Nucleotidase in Serum by Continuous Flow Analysis
| Autor: | J.H.M. Souverijn, W. van der Slik, J.-P. Persijn, W. C. H. van Helden |
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| Rok vydání: | 1980 |
| Předmět: |
Reproducibility
Autoanalysis Chromatography Correlation coefficient Adenosine Deaminase Chemistry Continuous flow education Biochemistry (medical) Clinical Biochemistry General Medicine 5'-nucleotidase Kinetics Incubation temperature Nucleotidases Nucleotidase Linear regression Humans 5'-Nucleotidase Automated method |
| Zdroj: | cclm. 18:333-338 |
| ISSN: | 1437-4331 1434-6621 |
| DOI: | 10.1515/cclm.1980.18.6.333 |
| Popis: | The manual method of Persijn & Van der Slik (J. Clin. Chem. Clin. Biochem. 6, 441-446 (1968): 7, 493-497 (1969); 8, 398-402 (1970) for the determination of serum 5'-nucleotidase has been adapted to the Auto-Analyzer II System. In the Auto-Analyzer II, the incubation temperature for the enzyme reactions is 37 degrees C, and the effective sample speed is 30/h, at a sample/wash ratio of 2:1. There is good agreement and correlation between the manual method and the Auto-Analyzer II method (equation of regression line: y = x + 0.6, correlation coefficient = 0.988; the normal range is 2.9-10.5 U/l for both methods). In routine use, within-run and between-day reproducibility are considerably smaller for the Auto-Analyzer II than for the manual method, especially when large numbers of samples are assayed. |
| Databáze: | OpenAIRE |
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