Determination of Flavonoids, Phenolic Acids, and Xanthines in Mate Tea (Ilex paraguariensis St.-Hil.)

Autor: Vicente Simon Haas, Željan Maleš, Darija Šarić, Mirza Bojić
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Zdroj: Journal of Analytical Methods in Chemistry, Vol 2013 (2013)
Journal of Analytical Methods in Chemistry
DOI: 10.1155/2013/658596
Popis: Raw material, different formulations of foods, and dietary supplements ofmatedemands control of the content of bioactive substances for which high performance thin layer chromatography (TLC), described here, presents simple and rapid approach for detections as well as quantification. Using TLC densitometry, the following bioactive compounds were identified and quantified: chlorogenic acid (2.1 mg/g), caffeic acid (1.5 mg/g), rutin (5.2 mg/g), quercetin (2.2 mg/g), and kaempferol (4.5 mg/g). The results obtained with TLC densitometry for caffeine (5.4 mg/g) and theobromine (2.7 mg/g) show no statistical difference to the content of total xanthines (7.6 mg/g) obtained by UV-Vis spectrophotometry. Thus, TLC remains a technique of choice for simple and rapid analysis of great number of samples as well as a primary screening technique in plant analysis.
Databáze: OpenAIRE