Low FAT4 expression is associated with a poor prognosis in gastric cancer patients
| Autor: | Xujun He, Jun-Gang Luo, Ji Xu, Ying-Jie Xia, Zhengchuang Liu, Hou-Quan Tao, Xiao-Ting Jiang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Metastasis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine distant metastasis FAT4 medicine Lymph node Gene knockdown Cell growth business.industry gastric cancer Cancer Methylation medicine.disease Demethylating agent 030104 developmental biology medicine.anatomical_structure Oncology chemistry 030220 oncology & carcinogenesis Cancer research Immunohistochemistry prognosis methylation business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | In this study, we investigated the role of Fat atypical cadherin 4 (FAT4) in gastric cancer (GC) progression. Immunohistochemical analysis showed lower FAT4 expression in tumor tissues from GC patients than in normal gastric epithelium. Lower FAT4 expression was associated with poor prognosis, tumor size and invasion, and lymph node and distant metastases. Multivariate analysis showed that TNM stage, lymph node and distant metastases, Lauren classification, and FAT4 expression were independent prognostic factors in GC. Methylation-specific PCR analysis showed increased FAT4 promoter methylation in GC tumor tissues and cell lines. Higher FAT4 promoter methylation was associated with low FAT4 expression and a poor prognosis. BGC-823 cells showed increased FAT4 expression upon treatment with 5-azacytidine, demethylating agent. FAT4 knockdown in BGC-823 cells led to increased cell proliferation, migration and invasiveness. Moreover, xenografts of BGC-823 cells with FAT4 knockdown showed enhanced tumor growth and metastasis in nude mice. These findings demonstrate that low FAT4 expression is associated with a poor prognosis in GC patients. |
| Databáze: | OpenAIRE |
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