A New Look at Etiological Factors of Idiopathic Scoliosis: Neural Crest Cells
| Autor: | Anton Strunov, Elena L Strokova, Elena Kiseleva, Alexander I. Shevchenko, Pavel P. Laktionov, Vladimir M. Subbotin, Lubov A Suldina, Alla M Zaydman |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Neurofilament Adolescent Embryonic Development Scoliosis Biology neurofilaments Pathogenesis 03 medical and health sciences Chondrocytes growth plate medicine Deformity the expression of proteins Humans Child scoliosis Embryogenesis Neural crest General Medicine medicine.disease Spine 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Neural Crest Neuromorphology chondrocyte Microscopy Electron Scanning Neuroglia synapses Female medicine.symptom Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Idiopathic scoliosis is one of the most common disabling pathologies of children and adolescents. Etiology and pathogenesis of idiopathic scoliosis remain unknown. To study the etiology of this disease we identified the cells' phenotypes in the vertebral body growth plates in patients with idiopathic scoliosis. Materials and methods: The cells were isolated from vertebral body growth plates of the convex and concave sides of the deformity harvested intraoperatively in 50 patients with scoliosis. Cells were cultured and identified by methods of common morphology, neuromorphology, electron microscopy, immunohistochemistry and PCR analysis. Results: Cultured cells of convex side of deformation were identified as chondroblasts. Cells isolated from the growth plates of the concave side of the deformation showed numerous features of neuro- and glioblasts. These cells formed synapses, contain neurofilaments, and expressed neural and glial proteins. Conclusion: For the first time we demonstrated the presence of cells with neural/glial phenotype in the concave side of the vertebral body growth plate in scoliotic deformity. We hypothesized that neural and glial cells observed in the growth plates of the vertebral bodies represent derivatives of neural crest cells deposited in somites due to alterations in their migratory pathway during embryogenesis. We also propose that ectopic localization of cells derived from neural crest in the growth plate of the vertebral bodies is the main etiological factor of the scoliotic disease. |
| Databáze: | OpenAIRE |
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