Prophylactic Antiarrhythmic Effect of Anesthetics at Subanesthetic Concentration on Epinephrine-Induced Arrhythmias in Rats after Brain Death
Autor: | Yuji Fujino, Yuka Miyata, Yukio Hayashi, Takahiko Kamibayashi, Masanori Sato, Mitsuo Iwasaki, Hiroo Yamanaka |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Male
Brain Death Article Subject Epinephrine Blotting Western Hemodynamics lcsh:Medicine Pharmacology General Biochemistry Genetics and Molecular Biology Sevoflurane Rats Sprague-Dawley medicine Animals Anesthetics General Immunology and Microbiology Dose-Response Relationship Drug business.industry lcsh:R Arrhythmias Cardiac General Medicine Dose–response relationship Isoflurane Anesthesia Connexin 43 Anesthetic Halothane business Propofol medicine.drug Research Article |
Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
ISSN: | 2314-6141 2314-6133 |
Popis: | The present study using brain death model of rats was designed to examine whether prophylactic administration of volatile anesthetics and propofol prevent the epinephrine-induced arrhythmias. A Fogarty catheter was placed intracranially for induction of brain death. After brain death, the rats were randomly assigned to five groups: the control group (no anesthetics), the sevoflurane group (0.8%), the isoflurane group (0.5%), the halothane group (0.3%), and the propofol group (195 μg·kg−1·min−1). These anesthetics were about 30% ofED50of each anesthetic. The arrhythmogenic dose of epinephrine was determined in each anesthetic group. In addition, we examined left ventricular levels of connexin 43 phosphorylation 30 min after administration of each anesthetic with Western blot analysis. The arrhythmogenic dose of epinephrine in the sevoflurane group was significantly higher than that in the control group, while the arrhythmogenic dose of epinephrine in any other anesthetic group was not different. On the other hand, the ratio of phosphorylated-connexin 43/total connexin 43 was also similar among the study groups. Thus, prophylactic administration of subanesthetic dose of sevoflurane is effective in preventing epinephrine-induced arrhythmias after brain death, but phosphorylation of connexin is not involved in the antiarrhythmic property of sevoflurane. |
Databáze: | OpenAIRE |
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