Arm Sequences Contribute to the Architecture and Catalytic Function of a λ Integrase-Holliday Junction Complex
| Autor: | Tom Ellenberger, Christine A. Shaw, Marco A. Azaro, Arthur Landy, Tapan K. Biswas, Marta Radman-Livaja |
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| Přispěvatelé: | Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) |
| Rok vydání: | 2003 |
| Předmět: |
Fluorescence Nucleic Acid Conformation Oligonucleotides/chemistry *Recombination
Time Factors Stereochemistry viruses Oligonucleotides Genetic Time Factors Catalysis 03 medical and health sciences chemistry.chemical_compound Tetramer Catalytic Domain Escherichia coli Fluorescence Resonance Energy Transfer Holliday junction [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology 030304 developmental biology Recombination Genetic Genetics 0303 health sciences Dose-Response Relationship Drug biology Oligonucleotide Bacteriophage lambda/*enzymology Catalysis Catalytic Domain DNA/*chemistry/genetics/metabolism Dose-Response Relationship Drug Escherichia coli/*metabolism Fluorescence Resonance Energy Transfer Microscopy 030302 biochemistry & molecular biology DNA Cell Biology Lambda phage biology.organism_classification Bacteriophage lambda Integrase DNA binding site Microscopy Fluorescence chemistry biology.protein Nucleic Acid Conformation Recombination |
| Zdroj: | Molecular Cell Molecular Cell, Elsevier, 2003, 11 (3), pp.783--94. ⟨10.1016/S1097-2765(03)00111-4⟩ |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/s1097-2765(03)00111-4 |
| Popis: | lambda integrase (Int) mediates recombination between attachment sites on lambda phage and E. coli DNAs. With the assistance of accessory proteins that induce DNA loops, Int bridges pairs of distinct arm- and core-type DNA binding sites to form synapsed recombination complexes, which then recombine via a Holliday junction (HJ) intermediate. We show that, in addition to promoting the proper positioning of Int protomers, the arm sequences facilitate the catalytic activities of the Int tetramer, independent of accessory proteins or physical continuity between the arm and core sites. We have determined the architecture of ternary complexes containing a HJ, Int, and P'1,2 arm-type DNA. These structures accommodate simultaneous binding of Int to direct-repeat arm sites and indirect-repeat core sites and afford a new view of the higher-order recombinogenic complexes. |
| Databáze: | OpenAIRE |
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