Characterization of peripheral blood TCR repertoire in patients with ankylosing spondylitis by high-throughput sequencing
| Autor: | Kai-Rong Lin, Wu Zuchang, Pan Yingming, Jin Yabin, Jin-Huan Cui, Mao Xiaofan, Xiang-Ping Chen, Wen-lue Lian, Dong-mei Guo, Wei Luo, Chuling Zhang, Lin Wei, Xiao Ping |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male T-Lymphocytes Immunology Autoimmunity Pilot Projects Human leukocyte antigen Disease Pathogenesis Cohort Studies 03 medical and health sciences Antigen Immunology and Allergy Medicine Humans Spondylitis Ankylosing Clonal Selection Antigen-Mediated Autoimmune disease Ankylosing spondylitis Blood Cells business.industry Repertoire T-cell receptor High-Throughput Nucleotide Sequencing General Medicine Biodiversity medicine.disease 030104 developmental biology Genes T-Cell Receptor beta Disease Progression Female business |
| Zdroj: | Human immunology. 79(6) |
| ISSN: | 1879-1166 |
| Popis: | Ankylosing spondylitis (AS) is a chronic and progressive autoimmune disease affecting the invasion of the spine, sacroiliac joints and peripheral joints. T cells play a vital role in the underlying pathogenesis of AS, which mediated autoimmune and inflammatory responses via specific recognition of autoantigen peptides presented by susceptibility HLA. Antigen-specific T cells triggered by HLA/antigen complexes will undergo a massive expansion that forming an uneven T cell repertoire. To enhance our understanding of T-cell-mediated autoimmune in AS, we applied TCR β chains high-throughput sequencing to AS patients for in-depth TCR repertoire analysis. A significantly lower TCR repertoire diversity was observed in peripheral blood of AS patients relative to controls. And severe patients in our AS cohort have a more restricted TCR repertoire than mild patients, suggesting that the TCR repertoire diversity might be associated with the clinical severity of disease. No V, J and VJ pairs with significant biased usage were identified, which indicated that the usage frequency deviation of certain V/J/V-J genes in AS patients is little. This is a pilot study with potentially interesting observation on reduced diversity of T cells repertoire in peripheral blood of AS patients and further studies are needed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect