Differential expression of genes in HepG2 cells caused by UC001kfo RNAi as shown by RNA-seq
| Autor: | Li-dan Chen, Tong Su, Tao Qin, Yan-feng Pan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biomedical Engineering Down-Regulation Biology Biochemistry Biomaterials Extracellular matrix 03 medical and health sciences 0302 clinical medicine RNA interference Genetics Humans RNA Messenger Cell adhesion Gene Messenger RNA Sequence Analysis RNA Hep G2 Cells Molecular biology Gene Expression Regulation Neoplastic Gene Ontology 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Cancer cell RNA Interference RNA Long Noncoding Signal transduction Signal Transduction |
| Zdroj: | Journal of Huazhong University of Science and Technology [Medical Sciences]. 37:510-515 |
| ISSN: | 1672-0733 |
| DOI: | 10.1007/s11596-017-1765-1 |
| Popis: | The differential expression of genes in HepG2 cells caused by UC001kfo RNAi was investigated using RNA-seq. HepG2 cells were infected by Lenti-shUC001kfo lentivirus particles. The expression of UC001kfo mRNA in the HepG2-shUC001kfo cell line was detected by real-time PCR. RNA-seq technology was used to identify the difference in the expression of genes regulated by lncRNA UC001kfo in the HepG2 cell line. Gene ontology and signaling pathway analysis were performed to reveal the biological functions of the genes encoding of significantly different mRNAs. The results showed that mRNAs were differentially expressed between the HepG2-shUC001kfo cell line and the HepG2 cell line. The UC001kfo mRNA was significantly down-regulated in the stable cell line HepG2-shUC001kfo (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink