Association of the placental VEGF promoter polymorphisms and VEGF mRNA expression with preeclampsia
| Autor: | Farshid Keshavarzi, Ava Rasooli, Minoo Yaghmaei, Mehrnaz Narooei-Nejad, Saeedeh Salimi, Mahnaz Shahrakipoor, Batool Teimoori |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Vascular Endothelial Growth Factor A medicine.medical_specialty Genotype Physiology Placenta 030204 cardiovascular system & hematology Real-Time Polymerase Chain Reaction Preeclampsia 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pre-Eclampsia Pregnancy Internal medicine Gene expression Internal Medicine medicine Humans 030212 general & internal medicine RNA Messenger Promoter Regions Genetic Gene reproductive and urinary physiology Messenger RNA Polymorphism Genetic business.industry fungi Case-control study General Medicine medicine.disease female genital diseases and pregnancy complications Up-Regulation Vascular endothelial growth factor Endocrinology medicine.anatomical_structure chemistry Case-Control Studies Female business |
| Zdroj: | Clinical and experimental hypertension (New York, N.Y. : 1993). 41(3) |
| ISSN: | 1525-6006 |
| Popis: | Preeclampsia (PE), is a pregnancy-specific complication with the placental origin which associated with altered expression of angiogenic factors. Vascular Endothelial Growth Factor (VEGF), is a growth and hypoxia-induced factor which contributes to the regulation of various processes. The present study has investigated the association of the placental VEGF -634G/C (rs2010963), -1154G/A (rs1570360), and -2549 I/D (18bpindel) polymorphisms in the promoter region with VEGF mRNA expression in PE women and control group.This case-control study was performed on the placenta of 84 PE women and 103 controls after delivery. Genotyping of the VEGF polymorphisms was done by PCR or PCR, PCR-RFLP or sequencing methods. The mRNA expression levels were measured by Quantitative Real-Time PCR.The relative mRNA expression of VEGF gene was significantly higher in PE women compared to controls. The relative mRNA expression of VEGF gene was significantly higher in women with -634CC genotype compared to CG + GG genotypes in PE women and total studied women but not in control women. However, there was no association between the placental VEGF -1154G/A and -2549 I/D polymorphisms and VEGF mRNA expression neither in PE nor in control groups.The current study found higher mRNA expression of placental VEGF gene in PE women. The mRNA expression of the placental VEGF gene has been up-regulated in the placenta of women with -634CC genotype. No association was found between the placental VEGF -1154G/A and -2549 I/D polymorphisms and VEGF mRNA expression. |
| Databáze: | OpenAIRE |
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