Brain lipid peroxidation and antioxidant levels in fetal lambs 72 hours after asphyxia by partial umbilical cord occlusion
| Autor: | Tomoaki Ikeda, Julian T. Parer, Shigeharu Doi, Edward J. Quilligan, Soung Day Park, Yuji Murata |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Thiobarbituric acid Brain damage medicine.disease_cause Fetal Hypoxia Thiobarbituric Acid Reactive Substances Statistics Nonparametric Umbilical Cord Superoxide dismutase chemistry.chemical_compound Internal medicine medicine Animals Umbilical Cord Occlusion Asphyxia Fetus Sheep biology business.industry Superoxide Dismutase Obstetrics and Gynecology Brain Constriction Glutathione Oxidative Stress Endocrinology chemistry biology.protein Base excess Female Lipid Peroxidation medicine.symptom business Oxidative stress |
| Zdroj: | American journal of obstetrics and gynecology. 178(3) |
| ISSN: | 0002-9378 |
| Popis: | OBJECTIVES: The purpose of this study was to explain the role of oxidative stress in the pathogenesis of brain damage caused by intrauterine fetal asphyxia. STUDY DESIGN: Six chronically instrumented near-term fetal lambs were subjected to asphyxia by partial umbilical cord occlusion for approximately 60 minutes until fetal arterial pH diminished to less than 6.9 and base excess to less than –20 mEq. Another six fetuses surgically prepared but not occluded were used as control. Fetuses were killed after 72 hours and eight different brain regions (frontal and parietal gray matter, frontal and parietal white matter, basal ganglia, thalamus, hippocampus, and cerebellum) were dissected and assayed for thiobarbituric acid reactive substances, glutathione, and superoxide dismutase. RESULTS: Thiobarbituric acid reactive substance levels in asphyxiated animals were elevated in frontal and parietal white matter, basal ganglia, and thalamus compared with those in controls. The concentrations of superoxide dismutase in the asphyxiated group were also higher in frontal and parietal white matter, basal ganglia, and cerebellum compared with those in the control group. Between the two groups, however, glutathione concentrations did not differ significantly. CONCLUSION: These results suggest that oxidative stress may be a major contributing factor to the development of brain damage in intrauterine fetal asphyxia. (Am J Obstet Gynecol 1998;178:474-8.) |
| Databáze: | OpenAIRE |
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