Cytokine-stimulated human immunodeficiency virus replication is inhibited by N-acetyl-L-cysteine
| Autor: | Roederer, M, Staal, F J, Raju, P A, Ela, S W, Herzenberg, L A |
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| Rok vydání: | 1990 |
| Předmět: |
Transcription
Genetic Recombinant Fusion Proteins medicine.medical_treatment Biology Pharmacology Virus Replication Antiviral Agents Virus Cell Line Acetylcysteine chemistry.chemical_compound medicine Humans Repetitive Sequences Nucleic Acid Multidisciplinary Tumor Necrosis Factor-alpha HIV beta-Galactosidase Virology Cytokine chemistry Viral replication Cell culture Phorbol Tetradecanoylphorbol Acetate Tumor necrosis factor alpha Intracellular Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 87:4884-4888 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | We show that the stimulation of human immunodeficiency virus (HIV) brought about by tumor necrosis factor alpha and phorbol 12-myristate 13-acetate can be inhibited by adding N-acetyl-L-cysteine (NAC). NAC, which replenishes intracellular glutathione, effectively inhibits the tumor necrosis factor alpha- or phorbol ester-stimulated replication of HIV in acutely infected cell cultures. NAC also inhibits the cytokine-enhanced HIV long terminal repeat-directed expression of beta-galactosidase in in vitro HIV model systems. These results show that intracellular thiol levels influence HIV production. Furthermore, because NAC reverses tumor necrosis factor alpha toxicity both in cells and in animals and is a well-known drug that can be administered orally without known toxicity in humans, these results suggest that NAC is a possible therapeutic agent in AIDS. |
| Databáze: | OpenAIRE |
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