Mitochondrial Channels Permeable by Calcium Ions

Autor:	Karol Ondrias, Tatiana Ravingerová, Marta Novotova, Adam Szewczyk, Viera Kominkova
Rok vydání:	2004
Předmět:	Ruthenium red Chemistry Ryanodine receptor Health Toxicology and Mutagenesis Inorganic chemistry Cardiac action potential Hyperpolarization (biology) Toxicology chemistry.chemical_compound Cyclosporin a Biophysics Ligand-gated ion channel Inner mitochondrial membrane Uniporter
Zdroj:	Toxicology Mechanisms and Methods. 14:35-39
ISSN:	1537-6524 1537-6516
DOI:	10.1080/15376520490257428
Popis:	The aim of this work was to characterize inner mitochondrial membrane channels permeable to calcium cations (Ca(++)). Mitochondrial membranes isolated from rat heart were incorporated into the bilayer lipid membrane, and single Ca(++) channel currents were measured. The observed channels were selective for Ca(++) and barium cations (Ba(++)) (53 and 50 mM) over Tris(+) (113 mM), but single-channel currents in most cases were noisy and did not show the typical single-channel shape, as it is known, for example, in the ryanodine receptor or in inositol 1,4,5-trisphosphate (IP(3)R) Ca(++)-release channels. The most commonly observed single-channel currents, measured at the 0 mV and 53 mM Ca(++) gradient, were in the range of 1 pA or less. The channels responded to pharmacological modulators. Some of the channels were inhibited by ruthenium red and cyclosporin A, and others were modulated by ryanodine. This may indicate that the observed channels passing Ca(++) may originate from the mitochondrial Ca(++) uniporter, the permeability transition pore, and the ryanodine receptor calcium channel.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c80322a6aecf2a57c08f56901b5bf195 https://doi.org/10.1080/15376520490257428 Zobrazit plný text záznamu Plný text ve formátu PDF