Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin-1, and endothelial dysfunction in streptozotocin treated rats
| Autor: | Lisa Lione, Christopher D. Benham, Rosemary Ugur, Yousif A. Shamsaldeen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Nitric Oxide Synthase Type III Endocrinology Diabetes and Metabolism Caveolin 1 030204 cardiovascular system & hematology Nitric Oxide Diabetes Mellitus Experimental 03 medical and health sciences 0302 clinical medicine Endocrinology Enos Internal medicine Diabetes mellitus Internal Medicine medicine Animals Vascular Diseases Endothelial dysfunction Rats Wistar Dyslipidemias Endothelial nitric oxide synthase biology business.industry medicine.disease biology.organism_classification Streptozotocin Rats Oxidative Stress 030104 developmental biology Hyperglycemia Endothelium Vascular business medicine.drug |
| Zdroj: | Diabetes/metabolism research and reviews. 34(5) |
| ISSN: | 1520-7560 |
| Popis: | Diabetes is a complex progressive disease characterized by chronic hyperglycaemia and dyslipidaemia associated with endothelial dysfunction. Oxidized LDL (Ox-LDL) is elevated in diabetes and may contribute to endothelial dysfunction. The aim of this study was to relate the serum levels of Ox-LDL with endothelial dysfunction in streptozotocin (STZ)-diabetic rats and to further explore the changes in endothelial nitric oxide synthase (eNOS) and caveolin-1 (CAV-1) expression in primary aortic endothelial cells.Diabetes was induced with a single intraperitoneal injection of STZ in male Wistar rats. During the hyperglycaemic diabetes state serum lipid markers, aortic relaxation and aortic endothelial cell eNOS and CAV-1 protein expressions were measured.Elevated serum Ox-LDL (STZ 1486 ± 78.1 pg/mL vs control 732.6 ± 160.6 pg/mL, P .05) was associated with hyperglycaemia (STZ 29 ± 0.9 mmol/L vs control: 7.2 ± 0.2 mmol/L, P .001) and hypertriglyceridaemia (STZ 9.0 ± 1.5 mmol/L vs control: 3.0 ± 0.3 mmol/L, P .01) in diabetic rats. A significant reduction was observed in STZ-diabetic aortic endothelial cell eNOS and CAV-1 of 40% and 30%, respectively, accompanied by a compromised STZ-diabetic carbachol-induced vasodilation (STZ 29.6 ± 9.3% vs control 77.2 ± 2.5%, P .001).The elevated serum Ox-LDL in hyperglycaemic STZ-diabetic rats may contribute to diabetic endothelial dysfunction, possibly through downregulation of endothelial CAV-1 and eNOS. |
| Databáze: | OpenAIRE |
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