Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood
| Autor: | Alberto José da Silva Duarte, Ernesto T. A. Marques, Orlando Piubelli, Liciana Xavier Eurico de Alencar, Thomas August, A. E. Fusaro, Milton Maciel, Cyro Alves de Brito, Adriana Letícia Goldoni, Maria Notomi Sato, Paula Ordonhez Rigato |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
DNA vaccine
Male T cell Immunization Secondary Priming (immunology) HIV Infections Biology Immunological memory DNA vaccination Mice Immune system Antigen Lysosomal-Associated Membrane Protein 1 T-Lymphocyte Subsets Neonatal Virology medicine Vaccines DNA Animals Protein Precursors B cell AIDS Vaccines Mice Inbred BALB C Vaccination CTL medicine.anatomical_structure Animals Newborn Immunology HIV-1 Female Immunization Immunologic Memory |
| Zdroj: | Virology. 406(1):37-47 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2010.06.050 |
| Popis: | Successful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-γ, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric LAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory. |
| Databáze: | OpenAIRE |
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