Potent, protective anti-HIV immune responses generated by bimodal HIV envelope DNA plus protein vaccination
| Autor: | John W. Shiver, Carol I. Lord, Norman L. Letvin, Mary-Ellen Davies, Daniel C. Freed, David C. Montefiori, Laurence K. Handt, Helen C. Perry, Margaret A. Liu, Marianne Alroy, Yasuhiro Yasutomi, Christine Lekutis |
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| Rok vydání: | 1997 |
| Předmět: |
Cytotoxicity
Immunologic Immunogen T-Lymphocytes viruses HIV Infections Simian Genes env Immune system Immunity Animals Humans Cytotoxic T cell Neutralizing antibody AIDS Vaccines Multidisciplinary biology Gene Products env virus diseases Haplorhini Biological Sciences biology.organism_classification Virology Vaccination Titer DNA Viral Immunology HIV-1 biology.protein Immunization Reassortant Viruses |
| Zdroj: | Proceedings of the National Academy of Sciences. 94:9378-9383 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.94.17.9378 |
| Popis: | It is generally thought that an effective vaccine to prevent HIV-1 infection should elicit both strong neutralizing antibody and cytotoxic T lymphocyte responses. We recently demonstrated that potent, boostable, long-lived HIV-1 envelope (Env)-specific cytotoxic T lymphocyte responses can be elicited in rhesus monkeys using plasmid-encoded HIV-1envDNA as the immunogen. In the present study, we show that the addition of HIV-1 Env protein to this regimen as a boosting immunogen generates a high titer neutralizing antibody response in this nonhuman primate species. Moreover, we demonstrate in a pilot study that immunization with HIV-1envDNA (multiple doses) followed by a final immunization with HIV-1envDNA plus HIV-1 Env protein (envgene from HXBc2 clone of HIV IIIB; Env protein from parental HIV IIIB) completely protects monkeys from infection after i.v. challenge with a chimeric virus expressing HIV-1env(HXBc2) on a simian immmunodeficiency virusmacbackbone (SHIV-HXBc2). The potent immunity and protection seen in these pilot experiments suggest that a DNA prime/DNA plus protein boost regimen warrants active investigation as a vaccine strategy to prevent HIV-1 infection. |
| Databáze: | OpenAIRE |
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