Flow rate and duty cycle effects in lysis ofChlamydomonas reinhardtiiusing high-energy pulsed focused ultrasound
| Autor: | Martin H. Spalding, Dan J. Stessman, Grant Riesberg, Timothy A. Bigelow, Linxing Yao, Tong Wang, Jin Xu |
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| Rok vydání: | 2014 |
| Předmět: |
Chlorophyll
Time Factors Lysis Acoustics and Ultrasonics Transducers Chlamydomonas reinhardtii Cell Fractionation High-Energy Shock Waves Motion Arts and Humanities (miscellaneous) Ultrasonics biology Pulse (signal processing) Chemistry business.industry Algal Proteins Ultrasound Equipment Design Lipid Metabolism biology.organism_classification Volumetric flow rate Transducer Duty cycle Biofuels Biophysics Energy source business |
| Zdroj: | The Journal of the Acoustical Society of America. 135:3632-3638 |
| ISSN: | 0001-4966 |
| DOI: | 10.1121/1.4874627 |
| Popis: | To consider microalgae lipid biofuel as a viable energy source, it is a necessity to maximize algal cell lysis, lipid harvest, and thus biofuel production versus the energy used to lyse the cells. Previous techniques have been to use energy consumptive ultrasound waves in the 10-40 kHz range in a stationary exposure environment. This study evaluated the potential of using 1.1 MHz ultrasound pulses in a new flow through type chamber on Chlamydomonas reinhardtii as a model organism for cell breakage. The ultrasound was generated using a spherically focused transducer with a focal length of 6.34 cm and an active diameter of 6.36 cm driven by 20 cycle sine-wave tone bursts at varied pulse repetition frequencies. First, variations in flow rate were examined at a constant duty cycle of 3.6%. After assessing flow rates, the duty cycle was varied to further explore the dependence on the tone burst parameters. Cell lysis was assessed by quantifying protein and chlorophyll release into the supernatant as well as by lipid extractability. Appropriate flow rates with higher duty cycles led to statistically significant increases in cell lysis relative to controls and other exposure conditions. |
| Databáze: | OpenAIRE |
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