Specific detection of avian pneumovirus (APV) US isolates by RT-PCR
| Autor: | David A. Halvorson, Hyun-Jin Shin, Kakambi V. Nagaraja, Sagar M. Goyal, Gireesh Rajashekara, Faris F. Jirjis, Daniel P. Shaw |
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| Rok vydání: | 2000 |
| Předmět: |
Turkeys
Pneumovirus Paramyxoviridae biology Reverse Transcriptase Polymerase Chain Reaction Nucleic acid sequence General Medicine biology.organism_classification Virology United States Reverse transcriptase Virus law.invention Avian pneumovirus Viral Matrix Proteins nervous system law Animals Pneumovirus Infections RNA Viral Primer (molecular biology) Poultry Diseases Polymerase chain reaction |
| Zdroj: | Archives of Virology. 145:1239-1246 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s007050070123 |
| Popis: | This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV). Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as low as 8 x 10(-5) TCID50 (0.0323 microg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from turkeys in Minnesota. |
| Databáze: | OpenAIRE |
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