Transcriptomics of bronchoalveolar lavage cells identifies new molecular endotypes of sarcoidosis
| Autor: | Alison Morris, Panayiotis V. Benos, Ronald G. Collman, Kevin F. Gibson, Milica Vukmirovic, Yingze Zhang, Heather Lynn, Xiting Yan, Buqu Hu, David R. Moller, Joe G.N. Garcia, Michael J. Becich, Giuseppe DeIuliis, Jonas C. Schupp, Laura L. Koth, Joseph K. Leader, S. R. Wisniewski, Taylor Adams, Edward S. Chen, Mridu Gulati, Antje Prasse, Lisa A. Maier, Erica L. Herzog, Harry Hochheiser, Nkiruka Emeagwali, Naftali Kaminski, Tony Woolard, Wonder P. Drake, Antun Mihaljinec |
|---|---|
| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pulmonary and Respiratory Medicine
Microarray Sarcoidosis Disease Bronchoalveolar Lavage Article Transcriptome Immune system Sarcoidosis Pulmonary Medicine Humans Mechanistic target of rapamycin Gene biology medicine.diagnostic_test business.industry Interleukin medicine.disease Phenotype Bronchoalveolar lavage Immunology biology.protein business Bronchoalveolar Lavage Fluid |
| Zdroj: | Eur Respir J |
| Popis: | Sarcoidosis is a multisystem granulomatous disease of unknown origin with a variable and often unpredictable course and pattern of organ involvement. In this study we sought to identify specific bronchoalveolar lavage (BAL) cell gene expression patterns indicative of distinct disease phenotypic traits.RNA sequencing by Ion Torrent Proton was performed on BAL cells obtained from 215 well characterized patients with pulmonary sarcoidosis enrolled in the multicenter Genomic Research in Alpha-1 Antitrypsin Deficiency and Sarcoidosis (GRADS) study. Weighted Gene Co-expression Network Analysis (WGCNA) and non-parametric statistics were used to analyze genome wide BAL transcriptome. Validation of results was performed using a microarray expression data set of an independent sarcoidosis cohort (Freiburg, Germany (n=50)).Our supervised analysis found associations between distinct transcriptional programs and major pulmonary phenotypic manifestations of sarcoidosis including; TH1 and TH17 pathways associated with hilar lymphadenopathy; TGFB1 and MTOR signaling with parenchymal involvement, and IL7 and IL2 with airway involvement. Our unsupervised analysis revealed gene modules that uncovered four potential sarcoidosis endotypes including hilar lymphadenopathy with increased acute T cell immune response; extraocular organ involvement with PI3K activation pathways; chronic and multiorgan disease with increased immune response pathways; and multiorgan with increased IL-1 and IL-18 immune and inflammatory responses. We validated the occurrence of these endotypes using gene expression, pulmonary function tests and cell differentials from Freiburg. Taken together our results identify BAL gene expression programs that characterize major pulmonary sarcoidosis phenotypes and suggest the presence of distinct disease molecular endotypes.Take home messageGenome wide BAL transcriptomics identified novel gene expression profiles associated with distinct phenotypic traits in sarcoidosis and is suggestive of the presence of novel molecular and clinical sarcoidosis endotypes that could help with further understanding of this heterogenous disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|