o-BENZOQUINONE-A REAGENT FOR DETERMINING CONFORMATIONAL DIFFERENCES IN RELATED PROTEINS

Autor:	G.S. Murthy, Munishwar N. Gupta, Paul J. Vithayathil
Rok vydání:	2009
Předmět:	Methionine Chemical Phenomena Protein Conformation Spectrum Analysis Quinones Hydrogen-Ion Concentration Biochemistry Benzoquinone Chemistry chemistry.chemical_compound Ribonucleases Protein structure chemistry Reagent Benzoquinones Organic chemistry Indicators and Reagents Amino Acids
Zdroj:	International Journal of Peptide and Protein Research. 15:243-247
ISSN:	0367-8377
Popis:	OBQ reacts specifically with methionine residues of RNAase A at highly acidic pH. At pH 3, whereas RNAase A does not react, RNAase S, S-protein and S-peptide all react with OBQ. RNAase A derivatives in which some of the methionines have been initially modified at pH 1, further react with OBQ at pH 3. Thus, reaction with OBQ can be used to monitor structural transitions and conformational differences involving methionines in proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7f865bb5213a1d17ddf5d1f2edb3de3 https://doi.org/10.1111/j.1399-3011.1980.tb02573.x Zobrazit plný text záznamu