Babesia bovis infection in cattle in the southwestern Brazilian Amazon
| Autor: | Rodrigo Barros Rocha, Thaís O.R.S. Falcoski, Luciana Correia de Almeida Regitano, Luciana G. Brito, Fabiano B. Vendrami, Gislaine C.R. Souza, Márcia Cristina de Sena Oliveira, Fabio da Silva Barbieri, Elisana Sales Ribeiro, Rodrigo Giglioti, Polyana C. Tizioto |
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| Rok vydání: | 2012 |
| Předmět: |
Cattle Diseases
Microbiology Polymerase Chain Reaction Sensitivity and Specificity law.invention law Babesiosis medicine Animals Polymerase chain reaction biology Babesia bovis DNA Protozoan medicine.disease biology.organism_classification Virology DNA extraction Infectious Diseases Insect Science Enzootic Parasitology Cattle Nested polymerase chain reaction Brazil Blood sampling |
| Zdroj: | Ticks and tick-borne diseases. 4(1-2) |
| ISSN: | 1877-9603 |
| Popis: | a b s t r a c t The present study provides the first epidemiological data on infection with Babesia bovis in cattle raised in the southwestern Brazilian Amazon. Blood clot samples were filtered through nylon cloth before being submitted to DNA extraction. PCR and nested-PCR were applied to assess the frequency of infection with B. bovis in calves with ages from 4 to 12 months bred in 4 microregions each in the states of Rondonia and Acre. After the DNA was extracted from the samples, the infection in cattle was investigated by amplification of the "rap1" gene from B. bovis. The DNA amplification results revealed a frequency of infection with B. bovis of 95.1% (272/286) in the samples from Rondonia and 96.1% (195/203) in those from Acre. The high frequency of B. bovis infection in the animals with ages from 4 to 12 months indicates a situation of enzootic stability in the regions studied. The infection rates are comparable to those detected by immunodiagnostic techniques in other endemic regions of Brazil. |
| Databáze: | OpenAIRE |
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