Cancer Cell Invasion of Mammary Organoids with Basal-In Phenotype
| Autor: | Celina G. Kleer, Sabra Djomehri, Kathryn E. Luker, David Mertz, Gary D. Luker, Hao Chen Chang, Soojung Lee, Jason Sentosa, Eric Parigoris, Madeleine Turner, Shuichi Takayama, Amy Y. Liu |
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| Rok vydání: | 2020 |
| Předmět: |
Biomedical Engineering
Pharmaceutical Science 02 engineering and technology 010402 general chemistry 01 natural sciences Basement Membrane Article Biomaterials Basal (phylogenetics) medicine Organoid Humans Neoplasm Invasiveness Basement membrane Chemistry Cancer Epithelial Cells 021001 nanoscience & nanotechnology medicine.disease Phenotype Epithelium 0104 chemical sciences Cell biology Organoids medicine.anatomical_structure Membrane Cancer cell 0210 nano-technology |
| Zdroj: | Adv Healthc Mater |
| ISSN: | 2192-2659 |
| Popis: | This paper describes mammary organoids with a basal-in phenotype where the basement membrane is located on the interior surface of the organoid. A key materials consideration to induce this basal-in phenotype is the use of a minimal gel scaffold that the epithelial cells self-assemble around and encapsulate. When MDA-MB-231 breast cancer cells are co-cultured with epithelial cells from day 0 under these conditions, cells self-organize into patterns with distinct cancer cell populations both inside and at the periphery of the epithelial organoid. In another type of experiment, the robust formation of the basement membrane on the epithelial organoid interior enables convenient studies of MDA-MB-231 invasion in a tumor progression-relevant direction relative to epithelial cell-basement membrane positioning. That is, the study of cancer invasion through the epithelium first, followed by the basement membrane to the basal side, is realized in an experimentally convenient manner where the cancer cells are simply seeded on the outside of pre-formed organoids, and their invasion into the organoid is monitored. Interestingly, invasion was more prominent when tumor cells were added to day 7 organoids with less developed basement membranes compared to day 16 organoids with more defined ones. |
| Databáze: | OpenAIRE |
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