In vivo hydroxyapatite scaffold performance in infected bone defects
| Autor: | Arpan Satsangi, Chun-Sik Bae, Kyu-Bok Lee, David D Dean, Nicholas Gerken, Mark R. Appleford, Jeffrey O. Hollinger, Joo L. Ong, Joseph J. Pearson, Teja Guda, Sean McBride |
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| Rok vydání: | 2018 |
| Předmět: |
Scaffold
medicine.medical_specialty Materials science Bone Regeneration medicine.drug_class medicine.medical_treatment 0206 medical engineering Antibiotics Biomedical Engineering 02 engineering and technology Bone and Bones Biomaterials In vivo Bone Density Drug Resistance Bacterial medicine Animals Regeneration Femur Autografts Bone mineral Wound Healing Bone Transplantation Tissue Engineering Tissue Scaffolds Histology Osteomyelitis X-Ray Microtomography 021001 nanoscience & nanotechnology 020601 biomedical engineering Surgery Durapatite Amputation Female Aseptic processing Rabbits 0210 nano-technology Porosity |
| Zdroj: | Journal of biomedical materials research. Part B, Applied biomaterialsREFERENCES. 108(3) |
| ISSN: | 1552-4981 |
| Popis: | Critically sized bone defects are often compounded by infectious complications. The standard of care consists of bone autografts with systemic antibiotics. These injuries and treatments lead to donor site morbidity, antibiotic resistant strains of bacteria, and often end stage amputation. This study proposes an alternative to the autograft using a porous, hydroxyapatite (HA) scaffold evaluated with and without infection and antibiotics. Twenty-four New Zealand white rabbits received either our HA scaffold or a pulverized autograft (PBA) within a surgically created critical-sized defect in the femur. The two grafts were evaluated in either septic or aseptic defects and with or without antibiotic treatment. The HA scaffolds were characterized with micro computed tomography. Post-euthanasia, micro computed tomography, histology, and white blood cells component analysis were completed. The HA had significantly greater (p < .001) mineralization to total volume than the PBA groups with 27.56% and 14.88%, respectively, and the septic HA groups were significantly greater than the aseptic groups both with and without antibiotics (p = .016). The bone quality denoted by bone mineral density was also significantly greater (p < .001) in the HA groups (67.01 ± 0.38 mgHA/cm3 ) than the PBA groups (64.66 ± 0.85 mgHA/cm3 ). The HA scaffold is a viable alternative to the bone autograft in defects with and without infection as shown by the quality and quantity of bone. |
| Databáze: | OpenAIRE |
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