Expression of CD10/neutral endopeptidase in normal and malignant tissues of the human stomach and colon
| Autor: | Naoaki Nakagawa, Yuji Hinoda, Yukihiko Sato, Fumio Itoh, Akira Yachi, Kohzoh Imai, Ryuzo Ueda, Yasuo Ohe |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.diagnostic_test
Stomach Calla Blotting Western Gastroenterology Biology Adenocarcinoma medicine.disease biology.organism_classification Molecular biology Immunohistochemistry Blot medicine.anatomical_structure Antigen Western blot Stomach Neoplasms hemic and lymphatic diseases Colonic Neoplasms medicine Carcinoma Tumor Cells Cultured Humans Neprilysin |
| Zdroj: | Journal of gastroenterology. 31(1) |
| ISSN: | 0944-1174 |
| Popis: | The expression of common acute lymphoblastic leukemia antigen (CALLA; CD10), which is identical to neutral endopeptidase (NEP, EC3.424.11), was examined in the malignant and adjacent noninvaded tissues of the human stomach and colon (n = 27). All of 27 normal and 18 well or moderately differentiated adenocarcioma tissue specimens were positive for monoclonal antibody (mAb) NL-1 against CD10/NEP, whereas the expression level was clearly decreased in all of 9 specimens of poorly differentiated adenocarcinoma. In addition, all of 7 gastric or colorectal carcinoma cell lines tested showed decreased expression of CD10/NEP. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis of the crude antigen J5 from the normal colon tissue lysate by mAb J5 detected a single band of approximately 100 kDa that was consistent with that of NALM-6 cells used as a positive control. These findings suggest that CD10/NEP is expressed in normal epithelial cells of the human stomach and colon, whereas the expression level is decreased in the poorly differentiated type of adenocarcinoma. |
| Databáze: | OpenAIRE |
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