Activation of a murine autoreactive B cell by immunization with human recombinant autoantigen La/SS-B: Characterization of the autoepitope
| Autor: | G. Pollak, W.J. van Venrooij, H. Bartsch, G.J.M. Pruijn, Helmut Tröster, Michael Bachmann, Imre Semsei, Martin Schwemmle, T.E. Metzger, R. Klein |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
medicine.drug_class
Recombinant Fusion Proteins Immunology Molecular Sequence Data Autoimmunity Biology Monoclonal antibody Autoantigens PC12 Cells Epitope law.invention Cell Line Mice Adenosine Triphosphate law medicine Immunology and Allergy Animals Humans Elméleti orvostudományok Amino Acid Sequence GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Peptide sequence chemistry.chemical_classification B-Lymphocytes Microscopy Confocal Linear epitope Orvostudományok 3T3 Cells Molecular biology Amino acid Rats chemistry Microscopy Fluorescence Ribonucleoproteins Recombinant DNA biology.protein Cattle Immunization Antibody Epitope Mapping Gene Deletion Conformational epitope |
| Zdroj: | Journal of Autoimmunity, 8, 6, pp. 825-842 Journal of Autoimmunity, 8, 825-842. Academic press ltd ResearcherID |
| ISSN: | 0896-8411 |
| Popis: | Immunization of Balb/c mice with a homogeneously purified recombinant human La/SS-B protein resulted in activation of an autoreactive B cell secreting a novel monoclonal anti-La antibody termed La4B6. La4B6 reacted with La protein from a variety of sources including human, bovine, rat and mouse. ATP blocked the binding of La4B6 to recombinant La protein. The human epitope was identified as consisting of the amino acid sequence SKGRRFKGKGKGN, which includes the proposed ATP-binding site of the La protein. In the human and bovine La protein, the epitope exists as a continuous amino acid sequence. In rat and mouse the epitope was found to consist of the amino acid sequence SKG interrupted by a species-specific insert of 16 amino acids, and followed by the second half of the epitope, the amino acid sequence RRFKGKGKGN. Our data suggest that in the case of the rat and mouse La proteins the two separated parts of the epitope are able to form a conformational epitope which looks similar to the continuous human epitope. |
| Databáze: | OpenAIRE |
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