Determination of Synthetic Cathinones in Urine Using Gas Chromatography-Mass Spectrometry Techniques
| Autor: | Hwei-Fang Cheng, Po-Yu Wang, Mei-Chih Lin, Hsiu-Kuan Chou, Ya-Chun Ko, Wei-Yin Hong, Yu-Pen Chen, Daniel Yang-Chih Shih, Lih-Ching Chiueh |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pyrrolidines
medicine.drug_class Health Toxicology and Mutagenesis Urinalysis Toxicology Gas Chromatography-Mass Spectrometry Analytical Chemistry Designer Drugs Methamphetamine Acetone chemistry.chemical_compound Limit of Detection medicine Ethylamines Environmental Chemistry Humans Solid phase extraction Benzodioxoles Butylone Derivatization 3 4-Methylenedioxyamphetamine Detection limit Chemical Health and Safety Chromatography Amphetamines Solid Phase Extraction Reproducibility of Results Synthetic Cathinone Designer drug Substance Abuse Detection chemistry Pentylone Calibration Gas chromatography Gas chromatography–mass spectrometry medicine.drug |
| Zdroj: | Journal of analytical toxicology. 40(1) |
| ISSN: | 1945-2403 |
| Popis: | In recent years, the abuse of synthetic cathinones has increased considerably. This study proposes a method, based on gas chromatography/mass spectrometry (GC-MS), to analyze and quantify six synthetic cathinones in urine samples: mephedrone (4-MMC), methylone (bk-MDMA), butylone, ethylone, pentylone and methylenedioxypyrovalerone (MDPV). In our procedure, the urine samples undergo solid-phase extraction (SPE) and derivatization prior to injection into the GC-MS device. Separation is performed using a HP-5MS capillary column. The use of selective ion monitoring (SIM mode) makes it is good sensitivity in this method, and the entire analysis process is within 18 min. In addition, the proposed method maintains linearity in the calibration curve from 50 to 2,000 ng/mL (r(2) > 0.995). The limit of detection of this method is 5 ng/mL, with the exception of MDPV (20 ng/mL); the limit of quantification is 20 ng/mL, with the exception of MDPV (50 ng/mL). In testing, the extraction performance of SPE was between 82.34 and 104.46%. Precision and accuracy results were satisfactory |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|