Immunoelectron Microscopic Detection of the Hepatitis B Virus Major Surface Protein in Dilated Perinuclear Membranes of Yeast Cells
| Autor: | R. Biemans, T. Cabezon, M. Dewilde, T. Rutgers, D. Thines, B. Petreparent |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
HBsAg
Hepatitis B Surface Antigens Lysis biology Immunoelectron microscopy Endoplasmic reticulum Saccharomyces cerevisiae Virion Cell Biology General Medicine Cell Transformation Viral Endoplasmic Reticulum Virus Replication biology.organism_classification Molecular biology Yeast Inclusion Bodies Viral Microbiology Polyclonal antibodies Genetics biology.protein Secretion Microscopy Immunoelectron Molecular Biology |
| Zdroj: | DNA and Cell Biology. 11:621-626 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.1992.11.621 |
| Popis: | The major surface protein of hepatitis B virus produced in Saccharomyces cerevisiae can be recovered from cell lysates in the form of 22-mm lipoprotein particles. Immunoelectron microscopy was applied to investigate site and time of particle assembly. Thin sections of yeast cells revealed that production of the S protein provoked a dilation of the endoplasmic reticulum. Dilated areas were specifically labeled with a polyclonal antibody raised against glutaraldehyde-treated yeast-derived HBsAg particles. In contrast to previous postulates of particle formation during cell lysis and extract preparation, these results suggest that particle formation in yeast occurs in the endoplasmic reticulum and that transport of particles along the secretion pathway is blocked. |
| Databáze: | OpenAIRE |
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