Apoptotic Cell Death and the Proliferative Capacity of Human Breast Cancers
| Autor: | Riccardo Graber, Gabriele A. Losa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Programmed cell death medicine.medical_treatment Estrogen receptor Apoptosis DNA fragmentation Breast Neoplasms Biology lcsh:RC254-282 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Progesterone receptor Carcinoma medicine Humans Neoplasm Invasiveness lcsh:QH573-671 030304 developmental biology Aged human breast cancer 0303 health sciences steroid hormone receptors lcsh:Cytology %S‐phase‐proliferative capacity Middle Aged medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Steroid hormone DNA ploidy chemistry 030220 oncology & carcinogenesis Cancer research Other DNA Cell Division |
| Zdroj: | Analytical Cellular Pathology : the Journal of the European Society for Analytical Cellular Pathology Analytical Cellular Pathology, Vol 16, Iss 1, Pp 1-10 (1998) |
| ISSN: | 1878-3651 0921-8912 |
| Popis: | The proliferative capacity (%S‐phase fraction), DNA ploidy, apoptosis frequency (DNA fragmentation) and steroid hormone receptor status (estrogen receptor, ER; progesterone receptor, PR) of 110 samples of human breast tissues with ductal invasive carcinoma were measured using biochemical and cytofluorimetric procedures. The DNA fragmentation had a left‐skewed frequency distribution and an overall median value of 1.64%, whilst the median %S‐phase fraction was 8%. The median %DNA fragmentation and %S‐phase fraction were 1.96% and 16% in hyperdiploid tumours (n=29; DNA index >1.1) higher than in hypodiploid tumors (n=10; DNA index 0.96), 0.38% and 7.5%. DNA diploid tumours (n=71) had median %DNA fragmentation and %S‐phase values of 1.68% and 6%, consistently lower than the median values of DNA hyperdiploid tumours. The ER content of hypodiploid tumours was about one half (median: 5.9 fmol/mg) the median values in hyperdiploid (10.6 fmol/mg) and diploid tumours (14.6 fmol/mg). This may correlate with the lowest frequency of apoptosis in hypodiploid tumours, at least when measured by biochemical methods which only detect cells in the late phases of apoptosis. In contrast, the median PR was lowest in hyperdiploid tumours than in hypo and/or diploid tumours. The %S‐phase/%fragmented DNA ratio for the hypodiploid tumours was 19.7, significantly higher than the ratios for hyperdiploid (8.2) and diploid tumours (3.6). These findings indicated that there is an imbalance between proliferative capacity and cell death or growth arrest in human breast tumours. This imbalance may well be linked to a loss of steroid hormone control. |
| Databáze: | OpenAIRE |
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