Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture
| Autor: | Myong-Chol Lee, Min-Jen Tseng, Ya-Hui Chi, Yu-Cheng Huang, Min-Lang Huang, Zhao-Ying Ding, Ying-Hsuan Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genotype Nuclear Envelope LINC complex Transfection Article Cell Line Animals Genetically Modified 03 medical and health sciences Transduction (genetics) 0302 clinical medicine Ovarian Follicle Animals Drosophila Proteins Humans Cytoskeleton Research Articles Regulation of gene expression biology Gene Expression Regulation Developmental Membrane Proteins Cell Biology biology.organism_classification Cell biology Drosophila melanogaster Phenotype 030104 developmental biology Microscopy Fluorescence Cytoplasm Multiprotein Complexes Female Signal transduction 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | LINC complexes connect the inner and outer nuclear membrane (ONM) to transduce nucleocytoskeletal force. Ding et al. identify an ONM protein, Kuduk/TMEM258, which modulates the quality of LINC complexes and regulates the nuclear envelope architecture, nuclear positioning, and the development of ovarian follicles. Linker of nucleoskeleton and cytoskeleton (LINC) complexes spanning the nuclear envelope (NE) contribute to nucleocytoskeletal force transduction. A few NE proteins have been found to regulate the LINC complex. In this study, we identify one, Kuduk (Kud), which can reside at the outer nuclear membrane and is required for the development of Drosophila melanogaster ovarian follicles and NE morphology of myonuclei. Kud associates with LINC complex components in an evolutionarily conserved manner. Loss of Kud increases the level but impairs functioning of the LINC complex. Overexpression of Kud suppresses NE targeting of cytoskeleton-free LINC complexes. Thus, Kud acts as a quality control mechanism for LINC-mediated nucleocytoskeletal connections. Genetic data indicate that Kud also functions independently of the LINC complex. Overexpression of the human orthologue TMEM258 in Drosophila proved functional conservation. These findings expand our understanding of the regulation of LINC complexes and NE architecture. |
| Databáze: | OpenAIRE |
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