Map positions of third chromosomal female sterile and lethal mutations of Drosophila melanogaster
| Autor: | Matthieu Cavey, Christopher Schuetze, Jia-Jen Duong, Paul Lasko, Beat Suter, Michelle Peters, Ruth E. DörigR.E. Dörig |
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| Rok vydání: | 2004 |
| Předmět: |
Ethyl methanesulfonate
Sterility Mutant Genes Insect medicine.disease_cause Chromosomes chemistry.chemical_compound Oogenesis Gene mapping Genetics medicine Animals Allele Molecular Biology Gene Alleles Mutation biology Ovary Chromosome Mapping General Medicine biology.organism_classification Drosophila melanogaster Phenotype chemistry Ethyl Methanesulfonate Female Genes Lethal Infertility Female Biotechnology |
| Zdroj: | Genome. 47(5) |
| ISSN: | 0831-2796 |
| Popis: | Chromosomal mutations induced by ethyl methanesulfonate (EMS) treatment can cause female sterility or maternal-effect lethality in Drosophila. EMS is particularly useful to researchers because it creates mutations independent of position effects. However, because researchers have little control over the chromosomal site of mutation, post-mutagenic genetic mapping is required to determine the cytological location of the mutation. To make a valuable set of mutants more useful to the research community, we have mapped the uncharacterized part of the female-sterile – maternal-effect lethal Tübingen collection. We mapped 49 female-sterile – maternal-effect lethal alleles and 72 lethal alleles to individual deficiency intervals on the third chromosome. In addition, we analyzed the phenotype of ovaries resulting from female sterile mutations. The observed phenotypes range from tumorous ovaries and early blocks in oogenesis, to later blocks, slow growth, blocks in stage 10, to apparently full development of the ovary. The mapping and phenotypic characterization of these 121 mutations provide the necessary information for the researcher to consider a specific mutant as a candidate for their gene of interest.Key words: Drosophila melanogaster, oogenesis, female sterile, maternal-effect lethal, EMS-induced mutations. |
| Databáze: | OpenAIRE |
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