Oncogenic transformation by equine herpesviruses (EHV) I. Properties of hamster embryo cells transformed by ultraviolet-irradiated EHV-1
| Autor: | Robin A. Robinson, Ronald G. Duff, Berch E. Henry, Dennis J. O'Callaghan |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Ultraviolet Rays
Cell Hamster Biology Antibodies Viral Virus Cell Line Cell membrane Cricetinae Virology medicine Animals Antigens Viral Herpesviridae Base Sequence Contact inhibition Cell Transformation Viral Molecular biology Clone Cells Cell Transformation Neoplastic medicine.anatomical_structure Cell culture Cytoplasm DNA Viral Sarcoma Experimental Oncovirus Herpesvirus 1 Equid |
| Zdroj: | Virology. 101:335-362 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(80)90449-3 |
| Popis: | Permissive primary hamster embryo cells (HEF) were morphologically transformed in vitro by uv-irradiated standard equine herpesivrus type 1 (EHV-1). Cell lines (LSEH) were established and were shown to exhibit growth and biological properties typical of transformed cells. These properties included an altered, network-like cellular morphology, loss of contact inhibition, increased saturation density, decreased generation time, immortality in culture, formation of colonies in soft agar, growth at low concentrations of serum, and resistance to EHV-1 superinfection. These LSEH transformed cell lines were oncogenic in both newborn and immunocompetent inbred LSH syngeneic hamsters, and LSEH tumor cell lines were established from the tumors which were identified as malignant fibrous sarcomas. Both the LSEH transformed and tumor cell lines were determined to be virus nonproducers, since released virus could be neither detected by electron microscopy, infectious center assays, or cocultivation assays nor induced by iododeoxyuridine (IUdR) or growth at higher temperature. Analysis of the hybridization kinetics of 125I- or 32P-labeled EHV-1 DNA with DNA from low-passaged LSEH transformed cell lines, tumor tissues, or LSEH tumor cell lines revealed that viral DNA sequences were present in all cells and that the amount of these viral DNA sequences varied from one cell line to another. In the transformed cell lines, these values ranged from 3.3 to 20.9% of the EHV-1 genome in amounts of 0.3 to 2.2 copies/cell, whereas the DNA of tumor tissues and low-passaged LSEH tumor cell lines contained from 2.2 to 12.2% of the EHV-1 genome in amounts of 1.2 to 4.0 copies/cell. These viral DNA sequences were expressed since viral antigens were detected in LSEH transformed cells, tumor tissues, and tumor cell lines. Neutralizing antibody, ND50 titers ranging from 1 8 to 1 100 , was detected in the sera of hamsters that had been inoculated with either LSEH transformed or LSEH tumor cells. In addition, EHV-1-specific speypeptides were demonstrated in the cell membrane, cytoplasm, and perinuclear region of both LSEH transformed and tumor cell lines by indirect immunofluorescence using antisera to purified EHV-1 virions or antisera to EHV-1 infected HEF cell extracts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|