The Local Anaesthetic Lignocaine Exhibits Potent Antilung Cancer Cell Activity by Inhibiting the Phosphoinositide 3-Kinases/Mammalian Target of Rapamycin/Mammalian Target of Rapamycin Pathway
| Autor: | Zhifu Mao, Quanling Dong |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Lung Neoplasms
Antineoplastic Agents Apoptosis Pharmacology 030226 pharmacology & pharmacy S Phase Phosphatidylinositol 3-Kinases 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Humans Anesthetics Local Phosphorylation Cytotoxicity Lung cancer PI3K/AKT/mTOR pathway Cell Proliferation Chemistry Kinase TOR Serine-Threonine Kinases Lidocaine General Medicine Cell cycle medicine.disease Blot Cancer cell 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Pharmacology. 104:139-146 |
| ISSN: | 1423-0313 0031-7012 |
| DOI: | 10.1159/000500743 |
| Popis: | We studied the effect of lignocaine (LIG) on lung cancer cells. LIG dose- and concentration-dependently reduced the viability of the lung cancer cell line 95D. Fluorescence microscopy revealed that LIG-induced apoptosis, and this was confirmed via flow cytometric analysis of cells treated with various concentrations of LIG; the drug increased the proportions of cells in S-phase. Bad and Bax levels rose, and that of Bcl2 fell significantly, after addition of LIG; Western blotting showed that the drug also reduced the levels of phosphorylated proteins involved in downstream phosphoinositide 3-kinases/mammalian target of rapamycin/mammalian target of rapamycin signaling. In conclusion, our results suggest that LIG may be a useful therapy for human lung carcinoma. |
| Databáze: | OpenAIRE |
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