beta-Lactamase substrate profiles of coagulase-negative skin staphylococci from orthopaedic inpatients and staff members
| Autor: | M. Thore |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Microbiology (medical)
Coagulase Micrococcaceae Staphylococcus Cefazolin Drug resistance Microbial Sensitivity Tests Nursing Staff Hospital medicine.disease_cause beta-Lactamases Microbiology medicine Cephaloridine Medical Staff Hospital Nitrocefin Humans biology General Medicine biology.organism_classification Infectious Diseases Orthopedics Phenotype Staphylococcus aureus Staphylococcal Skin Infections medicine.drug |
| Zdroj: | The Journal of hospital infection. 22(3) |
| ISSN: | 0195-6701 |
| Popis: | Three different beta-lactamase substrate profiles were identified in 95 isolates of coagulase-negative staphylococci (CNS), of 57 different phenotypes, from 16 orthopaedic inpatients and staff members in one ward, by applying a bacterial whole-cell assay based on the hydrolysis of cefazolin, cephaloridine and nitrocefin. The typability of the assay was 93%, and 91% of the CNS isolates could be classified. To assess the discrimination between the beta-lactamase profiles obtained in the whole-cell assay, beta-lactamase extracts from 19 of the CNS isolates were used for estimation of their relative beta-lactamase substrate affinity index (RSAI). The RSAI assay was able to type previously unclassifiable or nontypable isolates. Two of the profiles obtained with the whole-cell assay were similar to those of the Staphylococcus aureus controls producing A or D and B or C beta-lactamases respectively. The distribution of beta-lactamase substrate profiles among the CNS isolates indicated an efficient spread of these drug resistance genes. |
| Databáze: | OpenAIRE |
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