Photostable AIE probes for wash-free, ultrafast, and high-quality plasma membrane staining
| Autor: | Hao-Ran Jia, Arshad Khan, Liang Ma, Fu-Gen Wu, Imtiaz Hussain, Yao-Wen Jiang, Qian Ma, Xiaolin Lu, Ya-Xuan Zhu, Sayed Mir Sayed, Fei-Fei Yin |
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| Rok vydání: | 2021 |
| Předmět: |
Staining and Labeling
Chemistry Spectrum Analysis Cell Membrane Biomedical Engineering Hydrophobic moiety Imaging study General Chemistry General Medicine Plasma Cell Line Staining Membrane Molecular Probes Reagent Stilbenes Biophysics Humans General Materials Science Membrane staining Hydrophobic and Hydrophilic Interactions Ultrashort pulse |
| Zdroj: | Journal of Materials Chemistry B. 9:4303-4308 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/d1tb00049g |
| Popis: | Plasma membrane (PM), a fundamental building component of a cell, is responsible for a variety of cell functions and biological processes. However, it is still challenging to acquire its morphology and morphological variation information via an effective approach. Herein, we report a PM imaging study regarding an aggregation-induced emission luminogen (AIEgen) called tetraphenylethylene-naphthalimide+ (TPE-NIM+), which is derived from our previously reported tetraphenylethylene-naphthalimide (TPE-NIM). The designed AIEgen (TPE-NIM+) shows significant characteristics of ultrafast staining, high photostability, wash-free property, and long retention time at the PM, which can structurally be correlated with its positively charged quaternary amine and hydrophobic moiety. TPE-NIM+ is further applied for staining of different cell lines, proving its universal PM imaging capability. Most importantly, we demonstrate that TPE-NIM+ can clearly delineate the contours of densely packed living cells with high cytocompatibility. Therefore, TPE-NIM+ as a PM imaging reagent superior to currently available commercial PM dyes shall find a number of applications in the biological/biomedical fields and even beyond. |
| Databáze: | OpenAIRE |
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