Feline embryo development in commercially available human media supplemented with fetal bovine serum
| Autor: | Yasunori Tsujimoto, Ryoji Kanegi, Kikuya Sugiura, Jun Iwata, Noritoshi Kawate, Shingo Hatoya, Toshio Inaba, Md. Emtiaj Alam, Hiromichi Tamada, Kana Fujiki |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
040301 veterinary sciences Serum albumin Embryonic Development Fertilization in Vitro 0403 veterinary science Andrology Embryo Culture Techniques 03 medical and health sciences Human fertilization in vitro culture medicine Animals Humans Blastocyst Bovine serum albumin feline 030304 developmental biology 0303 health sciences fetal bovine serum General Veterinary biology Pronucleus Full Paper Chemistry Embryogenesis Embryo Serum Albumin Bovine 04 agricultural and veterinary sciences respiratory system equipment and supplies human medium Culture Media medicine.anatomical_structure embryonic structures biology.protein Cats Female sense organs Theriogenology Fetal bovine serum |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | Feline embryo development was examined for 7 days after fertilization using commercially available human media supplemented with 0.3% bovine serum albumin (BSA) or 5% fetal bovine serum (FBS). Cumulus-oocyte complexes were categorized as Grades 1, 2, and 3 according to morphology. Only-One Medium (OM) was used for in vitro culture (IVC) in OM + BSA, OM + FBS, and OM + BSA/FBS, with BSA supplementation for the first 2 days and FBS for the subsequent 5 days. Embryos cultured in Early Culture Medium (1-2 days) and Blastocyst Medium (3-7 days) were defined as EB + BSA and EB + BSA/FBS. The developmental rate until the blastocyst stage of Grade 1 and 2 oocytes cultured in OM + BSA/FBS was higher than for the other groups and was significantly higher than for the OM + BSA and EB + BSA groups (P |
| Databáze: | OpenAIRE |
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