Trypanosoma cruzi: biodistribution of technetium-99m pertechnetate in infected rats
| Autor: | Roseane Pereira da Silva, Vanessa Santos de Arruda Barbosa, Jeanete A. Moreira, Aldo Cunha Medeiros, Cecília Maria de Carvalho Xavier Holanda, Antônia Cláudia Jácome da Câmara, Daniel Pereira de Oliveira |
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| Rok vydání: | 2009 |
| Předmět: |
Chagas disease
Male Biodistribution Pathology medicine.medical_specialty Pertechnetate Trypanosoma cruzi Immunology chemistry.chemical_element Parasitemia Technetium chemistry.chemical_compound Random Allocation medicine Animals Chagas Disease Tissue Distribution Rats Wistar Radionuclide Imaging Gamma counter Sodium Pertechnetate Tc 99m biology General Medicine medicine.disease biology.organism_classification Molecular biology Blood proteins Rats Infectious Diseases chemistry Parasitology Radiopharmaceuticals |
| Zdroj: | Experimental parasitology. 123(4) |
| ISSN: | 1090-2449 |
| Popis: | With the aim of investigating the biodistribution of technetium-99m pertechnetate ((99m)TcO4-) in rats infected with Y strain of Tripanosoma Cruzi, at the peak of parasitemia, (14th day of infection), we injected Wistar rats with 0.1 ml of (99m)TcO4- (3.7MBq). After 60 min, the percentage of radioactivity per gram was counted in several isolated organs and blood, using a gamma counter (1470 Wizard, PerkinElmer Finland). The uptake of (99m)TcO4- increased significantly in blood and decreased in the colon of infected animals (p0.05). A significant reduction in serum iron and red blood cells and a significant increase in total proteins, leukocytes and lymphocytes in the infected rats were observed, compared with controls (p0.05). A reduction in muscle layer thickness of the colon and mononuclear inflammation were observed. These results conclusively demonstrate that T. cruzi infection would be associated with changes in the biodistribution of (99m)TcO4- and in colon morphology, with potential clinical implications. |
| Databáze: | OpenAIRE |
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