EStradiol and PRogesterone in In vitro ferTilization (ESPRIT): a multicenter study evaluating third- versus second-generation estradiol and progesterone immunoassays
| Autor: | N P, Polyzos, E, Anckaert, P, Drakopoulos, H, Tournaye, J, Schiettecatte, H, Donner, G, Bobba, G, Miles, W D J, Verhagen-Kamerbeek, E, Bosch |
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| Přispěvatelé: | Faculty of Medicine and Pharmacy, Pathology/molecular and cellular medicine, Clinical Biology, Follicle Biology, Surgical clinical sciences, Centre for Reproductive Medicine - Gynaecology, Biology of the Testis, Faculty of Economic and Social Sciences and Solvay Business School, Faculty of Arts and Philosophy |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Adolescent Endocrinology Diabetes and Metabolism Blood Chemical Analysis/methods Fertilization in Vitro Immunoassay/methods 03 medical and health sciences 0302 clinical medicine Endocrinology In vitro fertilization tandem mass spectrometry Humans Progesterone/analysis Estradiol/analysis Progesterone Monitoring Physiologic Retrospective Studies Immunoassay 030219 obstetrics & reproductive medicine Estradiol Correction Middle Aged 030104 developmental biology Monitoring Physiologic/methods young adult Female ovulation induction Original Article Ovarian stimulation Blood Chemical Analysis Chromatography Liquid |
| Zdroj: | Journal of Endocrinological Investigation J Endocrinol Invest |
| ISSN: | 1720-8386 0391-4097 |
| Popis: | Purpose To assess estradiol (E2) and progesterone levels during ovarian stimulation determined by third-generation (Gen III) and second-generation (Gen II) Elecsys® immunoassays. Methods E2 and progesterone concentrations were measured using Elecsys® Gen III and Gen II immunoassays, and progesterone concentrations on the day of ovulation triggering were determined by LC–MS/MS. This was a retrospective, non-interventional study conducted at European tertiary referral infertility clinics in women aged 18–45 years, with a body mass index 18–35 kg/m2, regular menses, and both ovaries. Results Serum samples were obtained from 230 women classified by oocyte retrieval as poor (33.0%; 0–3 oocytes), normal (40.9%; 4–15 oocytes), or high (26.1%; > 15 oocytes) responders. E2 and progesterone levels increased during ovarian stimulation, with greatest increases observed in high responders. Elecsys® Gen III and Gen II assay results were highly correlated for E2 (Pearson’s r = 0.99) and progesterone (r = 0.89); Gen III results were lower than Gen II for both E2 and progesterone. On the day of triggering, Gen III E2 and progesterone levels showed a difference of − 15.0% and − 27.9%, respectively. Progesterone levels (on day of triggering) measured by LC–MS/MS correlated better with Gen III (0.98) than Gen II (0.90). Mean relative differences for Gen III and Gen II assays versus LC–MS/MS were 14.6% and 62.8%, respectively. Conclusion E2 and progesterone levels determined with Elecsys® Gen II and III assays were highly correlated; results were lower for Gen III versus Gen II. Differences observed for progesterone on the day of triggering may be clinically relevant. |
| Databáze: | OpenAIRE |
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