Capsanthin Inhibits both Adipogenesis in 3T3-L1 Preadipocytes and Weight Gain in High-Fat Diet-Induced Obese Mice
| Autor: | Hye Ok Choi, Sun Hee Woo, Byung Hoon Han, Jeung Won Kim, Hyung Joong Kim, Jung Hwan Kim, Sung Jun Jo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Adrenergic receptor medicine.medical_treatment 030209 endocrinology & metabolism Capsanthin Biochemistry Spontaneous locomotive activity 03 medical and health sciences 0302 clinical medicine Internal medicine Drug Discovery medicine Pharmacology Adiponectin Chemistry Insulin High fat diet 3T3-L1 Biological activity Anti-adipogenic Lypolytic activity 030104 developmental biology Endocrinology Adipogenesis Molecular Medicine Original Article medicine.symptom Weight gain |
| Zdroj: | Biomolecules & Therapeutics |
| ISSN: | 1976-9148 2005-4483 |
| DOI: | 10.4062/biomolther.2017.048 |
| Popis: | Adipogenesis in murine preadipocyte 3T3L-1 has been used as a model system to study anti-obese bioactive molecules. During adipogenesis in 3T3-L1 preadipocytes, we found that capsanthin inhibited adipogenesis (IC50; 2.5 μM) and also showed lipolytic activity in differentiated adipocytes from the preadipocytes (ED50; 872 nM). We identified that the pharmacological activity of capsanthin on adipogenesis in 3T3-L1 was mainly due to its adrenoceptor-β2-agonistic activity. In high-fat diet animal model study, capsanthin significantly enhanced spontaneous locomotive activities together with progressive weight-loss. The capsanthin-induced activation of kinetic behavior in mice was associated with the excessive production of ATP initiated by both the enhanced lipolytic activity together with accelerated oxidation of fatty acids due to the adrenoceptor β2-agonistic activity of capsanthin. Capsanthin also dose-dependently increased adiponectin and p-AMPK activity in high fat diet animals, suggesting that capsanthin has both anti-obesity and insulin sensitizing activities. |
| Databáze: | OpenAIRE |
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