Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia

Autor: Guangbin Zhu, Dong Chen, Zhonghua Yang, Changying Xie, Yongzhi Wang, Xinghuan Wang
Rok vydání: 2018
Předmět:
Zdroj: Biomedical Reports.
ISSN: 2049-9442
2049-9434
DOI: 10.3892/br.2018.1089
Popis: The present study aimed to determine whether the expression of transient receptor potential channel 5 (TRPC5) protein is altered in spermatozoa of patients with varicocele-associated asthenozoospermia. TRPC5 expression in spermatozoa was determined by polymerase chain reaction and western blotting analyses, and indirect immunofluorescence was used for identification and immunolocalization of the TRPC5 channel in human sperm. Sperm motility and superoxide dismutase (SOD) activity were also determined with a computer-assisted semen analysis system and assay kit, respectively. Compared with levels in control subjects, it was identified that TRPC5 protein expression, SOD activity and cellular motility in the sperm of patients with varicocele-associated asthenozoospermia were reduced (P
Databáze: OpenAIRE