Expression of TRPC5 is decreased in the sperm of patients with varicocele‑associated asthenozoospermia
Autor: | Guangbin Zhu, Dong Chen, Zhonghua Yang, Changying Xie, Yongzhi Wang, Xinghuan Wang |
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Rok vydání: | 2018 |
Předmět: |
endocrine system
040301 veterinary sciences Varicocele Semen analysis TRPC5 Asthenozoospermia General Biochemistry Genetics and Molecular Biology Male infertility 0403 veterinary science Superoxide dismutase Andrology medicine General Pharmacology Toxicology and Pharmaceutics Sperm motility medicine.diagnostic_test biology urogenital system Chemistry General Neuroscience 0402 animal and dairy science Articles 04 agricultural and veterinary sciences General Medicine medicine.disease 040201 dairy & animal science Sperm biology.protein |
Zdroj: | Biomedical Reports. |
ISSN: | 2049-9442 2049-9434 |
DOI: | 10.3892/br.2018.1089 |
Popis: | The present study aimed to determine whether the expression of transient receptor potential channel 5 (TRPC5) protein is altered in spermatozoa of patients with varicocele-associated asthenozoospermia. TRPC5 expression in spermatozoa was determined by polymerase chain reaction and western blotting analyses, and indirect immunofluorescence was used for identification and immunolocalization of the TRPC5 channel in human sperm. Sperm motility and superoxide dismutase (SOD) activity were also determined with a computer-assisted semen analysis system and assay kit, respectively. Compared with levels in control subjects, it was identified that TRPC5 protein expression, SOD activity and cellular motility in the sperm of patients with varicocele-associated asthenozoospermia were reduced (P |
Databáze: | OpenAIRE |
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