Central carbon metabolism ofSaccharomyces cerevisiaein anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions
| Autor: | Eija Rintala, Merja Penttilä, Laura Ruohonen, Paula Jouhten, Helena Simolin, Anne Huuskonen, Anu Tamminen, Jari Kiuru, Laura Salusjärvi, Raimo A. Ketola, Hannu Maaheimo, Marilyn G. Wiebe, Juha Kokkonen, Mervi Toivari |
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| Rok vydání: | 2008 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Metabolite Citric Acid Cycle chemistry.chemical_element Saccharomyces cerevisiae Biology Applied Microbiology and Biotechnology Microbiology Oxygen 03 medical and health sciences chemistry.chemical_compound Hypoxic transient Gene Expression Regulation Fungal Metabolites Glycolysis Anaerobiosis 030304 developmental biology 0303 health sciences 030306 microbiology General Medicine Metabolism Aerobiosis Carbon Culture Media Citric acid cycle Glucose Gene transcription chemistry Biochemistry Anaerobic glycolysis Energy Metabolism Systems biology Phosphoenolpyruvate carboxykinase Anaerobic exercise Metabolic Networks and Pathways |
| Zdroj: | Wiebe, M G, Rintala, E, Tamminen, A, Simolin, H, Salusjärvi, L, Toivari, M, Kokkonen, J T, Kiuru, J, Ketola, R A, Jouhten, P, Huuskonen, A, Maaheimo, H, Ruohonen, L & Penttilä, M 2008, ' Central carbon metabolism of Saccharomyces cerevisiae in anaerobic, oxygen-limited and fully aerobic steady-state conditions and following a shift to anaerobic conditions ', FEMS Yeast Research, vol. 8, no. 1, pp. 140-154 . https://doi.org/10.1111/j.1567-1364.2007.00234.x |
| ISSN: | 1567-1364 1567-1356 |
| DOI: | 10.1111/j.1567-1364.2007.00234.x |
| Popis: | Saccharomyces cerevisiae CEN.PK113-1A was grown in glucose-limited chemostat culture with 0%, 0.5%, 1.0%, 2.8% or 20.9% O2 in the inlet gas (D=0.10 h-1, pH 5, 30°C) to determine the effects of oxygen on 17 metabolites and 69 genes related to central carbon metabolism. The concentrations of tricarboxylic acid cycle (TCA) metabolites and all glycolytic metabolites except 2-phosphoglycerate+3-phosphoglycerate and phosphoenolpyruvate were higher in anaerobic than in fully aerobic conditions. Provision of only 0.5-1% O2 reduced the concentrations of most metabolites, as compared with anaerobic conditions. Transcription of most genes analyzed was reduced in 0%, 0.5% or 1.0% O2 relative to cells grown in 2.8% or 20.9% O 2. Ethanol production was observed with 2.8% or less O2. After steady-state analysis in defined oxygen concentrations, the conditions were switched from aerobic to anaerobic. Metabolite and transcript levels were monitored for up to 96 h after the transition, and this showed that more than 30 h was required for the cells to fully adapt to anaerobiosis. Levels of metabolites of upper glycolysis and the TCA cycle increased following the transition to anaerobic conditions, whereas those of metabolites of lower glycolysis generally decreased. Gene regulation was more complex, with some genes showing transient upregulation or downregulation during the adaptation to anaerobic conditions. |
| Databáze: | OpenAIRE |
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