Fatty acid-induced tuberculocidal activity in sera of guinea pigs treated with bacillus Calmette-Guérin and lipopolysaccharide
| Autor: | Michael Berendt, Ivan Kochan |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Lipopolysaccharides
Blood Bactericidal Activity Immunodiffusion Chromatography Gas Lipopolysaccharide Linoleic acid Iron Guinea Pigs Caviidae Oleic Acids Palmitic Acids Microbiology chemistry.chemical_compound Albumins Alkanes Immunology and Allergy Animals Sodium Hydroxide chemistry.chemical_classification Mycobacterium bovis biology Immune Sera Fatty Acids Albumin Fatty acid Mycobacterium tuberculosis biology.organism_classification Infectious Diseases chemistry Linoleic Acids BCG Vaccine BCG vaccine Mycobacterium |
| Zdroj: | The Journal of infectious diseases. 129(6) |
| ISSN: | 0022-1899 |
| Popis: | This study showed that sera of guinea pigs immunologically activated by vaccination and by challenge with bacillus Calmette-Gu6rin (BCG) and sera of lipopolysaccharide (LPS)-treated guinea pigs have tuberculocidal activity. Like the tuberculocidal activity of oleic acid, the serum tuberculocidal activity was not neutralized by the addition of exogenous iron, but it was neutralized quite effectively by the addition of albumin. Both BCG- or LPS-induced tuberculocidal activities disappeared in about one week after treatment. Titrimetric determinations and gas-liquid chromatographic studies showed that the antibacterial activity in sera of immunologically stimulated animals is caused by the increased levels of myristic, palmitic, oleic, and linoleic acids. These fatty acids were found to be highly toxic for the BCG strain of Mycobacterium bovis. The tuberculocidal effect in sera of immunologically activated animals increases according to the number of previous vaccinations. This finding indicates that the antituberculous activity in serum is an expression of immunologic processes that take place in the body of sensitive animals during severe immunologic reactions. Some strains of Mycobacterium are unable to grow |
| Databáze: | OpenAIRE |
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