RNAi-mediated germline knockdown of FABP4 increases body weight but does not improve the deranged nutrient metabolism of diet-induced obese mice
| Autor: | Kenneth P. Ellsworth, M A Cleary, Gino Castriota, T F Vogt, Margaret Wu, Ruojing Yang, S Xu, Bei Zhang, Xiaodong Yang, M K Shin, Sajjad A. Qureshi, Yiping Chen, J-Lv Tran, Joel P. Berger |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism medicine.medical_treatment Mice Obese Medicine (miscellaneous) Fatty Acid-Binding Proteins fatty acids lipids Mice chemistry.chemical_compound Insulin resistance RNA interference insulin resistance Internal medicine Adipocyte Adipocytes medicine Gene Knockdown Techniques Animals Obesity RNA Messenger Germ-Line Mutation Mice Knockout Gene knockdown Nutrition and Dietetics Chemistry Insulin Body Weight Lipid metabolism Lipid Metabolism medicine.disease Neoplasm Proteins Endocrinology glucose intolerance Original Article RNA Interference Energy Intake Energy Metabolism Diet-induced obese |
| Zdroj: | International Journal of Obesity (2005) |
| ISSN: | 1476-5497 0307-0565 |
| DOI: | 10.1038/ijo.2010.128 |
| Popis: | Objective: To investigate the impact of reduced adipocyte fatty acid-binding protein 4 (FABP4) in control of body weight, glucose and lipid homeostasis in diet-induced obese (DIO) mice. Methods: We applied RNA interference (RNAi) technology to generate FABP4 germline knockdown mice to investigate their metabolic phenotype. Results: RNAi-mediated knockdown reduced FABP4 mRNA expression and protein levels by almost 90% in adipocytes of standard chow-fed mice. In adipocytes of DIO mice, RNAi reduced FABP4 expression and protein levels by 70 and 80%, respectively. There was no increase in adipocyte FABP5 expression in FABP4 knockdown mice. The knockdown of FABP4 significantly increased body weight and fat mass in DIO mice. However, FABP4 knockdown did not affect plasma glucose and lipid homeostasis in DIO mice; nor did it improve their insulin sensitivity. Conclusion: Our data indicate that robust knockdown of FABP4 increases body weight and fat mass without improving glucose and lipid homeostasis in DIO mice. |
| Databáze: | OpenAIRE |
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